標題: alpha B-Crystallin in clear cell renal cell carcinoma: Tumor progression and prognostic significance
作者: Ho, Pei-Yin
Chueh, Shih-Chieh
Chiou, Shyh-Horng
Wang, Shuo-Meng
Lin, Wei-Chou
Lee, I-Liang
Yang, Hsin-Yi
Peng, Hsiang-Chi
Lai, Ming-Kuen
生物科技學系
Department of Biological Science and Technology
關鍵字: Clear cell renal cell carcinoma;alpha B-crystallin;Prognostic factor;Proliferation;Migration;Drug resistance
公開日期: 1-Oct-2013
摘要: Objectives: AlphaB-crystallin (alpha B-crystallin), a small heat shock protein, has been reported to be involved in the growth, antiapoptosis, migration, and chemoresistance of human malignancies. Materials and methods: alpha B-crystallin expression in normal renal and clear cell renal cell carcinoma (ccRCC) tissues was examined with two-dimensional (2D) gel electrophoresis assays. Immunohistochemistry was conducted to determine the presence of alpha B-crystallin-positive tumor cells and staining intensity in 50 cases of ccRCC tissue samples. The association of alpha B-crystallin protein expression, clinicopathogic parameters and prognosis of ccRCC patients was also analyzed with Student's t-test and Kaplan-Meier analysis. Moreover, Western blot assays were performed to detect the protein expression of alpha B-crystallin in normal and tumor tissues and the alteration of cell cycle regulators in alpha B-crystallin-overexpressing cells. MTT (3[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide), BrdU, and transwell assays were performed to demonstrate the effects of aB-crystallin overexpression on cell growth, DNA synthesis and cell migration of ccRCC cells, respectively. Results: The results showed the up-regulation of alpha B-crystallin expression in ccRCC tissues. Overall survival of ccRCC patients was significantly correlated with alpha B-crystallin expression in tumor tissues. We found that aB-crystallin overexpression increased the expression of cyclin A and the incorporation of BrdU, which may be related to the enhancement of cell growth. Transwell analyses demonstrated that presence of alpha B-crystallin overexpression enhanced cell migration in ccRCC cells. Furthermore, rapamycin-resistance of tumor cells was induced when alpha B-crystallin was overexpressed. Conclusions: Our experimental findings highlight the importance of alpha B-crystallin in the tumor growth, migration, and target therapy-resistance of ccRCC cells. (C) 2013 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.urolonc.2012.01.015
http://hdl.handle.net/11536/23016
ISSN: 1078-1439
DOI: 10.1016/j.urolonc.2012.01.015
期刊: UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
Volume: 31
Issue: 7
起始頁: 1367
結束頁: 1377
Appears in Collections:Articles


Files in This Item:

  1. 000325664300058.pdf

If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.