Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kuo, SY | en_US |
dc.contributor.author | Chien, LF | en_US |
dc.contributor.author | Hsiao, YY | en_US |
dc.contributor.author | Van, RC | en_US |
dc.contributor.author | Yan, KH | en_US |
dc.contributor.author | Liu, PF | en_US |
dc.contributor.author | Mao, SJ | en_US |
dc.contributor.author | Pan, RL | en_US |
dc.date.accessioned | 2014-12-08T15:35:03Z | - |
dc.date.available | 2014-12-08T15:35:03Z | - |
dc.date.issued | 2005-02-01 | en_US |
dc.identifier.issn | 0176-1617 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.jplph.2004.07.007 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/23799 | - |
dc.description.abstract | Endoplasmic reticulurn (ER)-enriched vesicles from etiolated hypocotyts of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition. The ER-enriched vesicles contained inorganic pyrophosphate (PPi) hydrolysis and its associated proton translocating activities. Antiserum prepared against vacuolar H+-pyrophosphatase (V-PPase, EC 3.6.1.1) did not inhibit this novel pyrophosphatase-dependent proton translocation, excluding the possible contamination of tonoptast vesicles in the ER-enriched membrane preparation. The optimal ratios of Mg2+/PPi (inorganic pyrophosphate) for enzymatic activity and PPj-dependent proton translocation of ER-enriched vesicles were, higher than those - of vacuolar membranes. The PPj-dependent Proton translocation of ER-enriched vesicles absolutely required the presence of monovalent cations with preference for K but could be inhibited by a common PPase inhibitor, F-. Furthermore, ER H+-pyrophosphatase exhibited some similarities and differences to vacuolar H+-PPases in cofactor/substrate ratios, pH profile, and concentration dependence of F-, imidodiphosphate (a PPj anatogue), and various chemical modifiers. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | endoplasmic reticulum | en_US |
dc.subject | H+-pyrophosphatase | en_US |
dc.subject | proton translocation | en_US |
dc.subject | tonoplast | en_US |
dc.subject | vacuole | en_US |
dc.title | Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.jplph.2004.07.007 | en_US |
dc.identifier.journal | JOURNAL OF PLANT PHYSIOLOGY | en_US |
dc.citation.volume | 162 | en_US |
dc.citation.issue | 2 | en_US |
dc.citation.spage | 129 | en_US |
dc.citation.epage | 138 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000227721100001 | - |
dc.citation.woscount | 4 | - |
Appears in Collections: | Articles |
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