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dc.contributor.authorOkuno, Masanarien_US
dc.contributor.authorKano, Hideakien_US
dc.contributor.authorFujii, Kenkichien_US
dc.contributor.authorBito, Kotatsuen_US
dc.contributor.authorNaito, Satoruen_US
dc.contributor.authorLeproux, Philippeen_US
dc.contributor.authorCouderc, Vincenten_US
dc.contributor.authorHamaguchi, Hiro-oen_US
dc.date.accessioned2014-12-08T15:35:52Z-
dc.date.available2014-12-08T15:35:52Z-
dc.date.issued2014-04-07en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0093401en_US
dc.identifier.urihttp://hdl.handle.net/11536/24245-
dc.description.abstractThe mechanism of surfactant-induced cell lysis has been studied with quantitative coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The dynamics of surfactant molecules as well as intracellular biomolecules in living Chinese Hamster Lung (CHL) cells has been examined for a low surfactant concentration (0.01 w%). By using an isotope labeled surfactant having CD bonds, surfactant uptake dynamics in living cells has been traced in detail. The simultaneous CARS imaging of the cell itself and the internalized surfactant has shown that the surfactant molecules is first accumulated inside a CHL cell followed by a sudden leak of cytosolic components such as proteins to the outside of the cell. This finding indicates that surfactant uptake occurs prior to the cell lysis, contrary to what has been believed: surface adsorption of surfactant molecules has been thought to occur first with subsequent disruption of cell membranes. Quantitative CARS microspectroscopy enables us to determine the molecular concentration of the surfactant molecules accumulated in a cell. We have also investigated the effect of a drug, nocodazole, on the surfactant uptake dynamics. As a result of the inhibition of tubulin polymerization by nocodazole, the surfactant uptake rate is significantly lowered. This fact suggests that intracellular membrane trafficking contributes to the surfactant uptake mechanism.en_US
dc.language.isoen_USen_US
dc.titleSurfactant Uptake Dynamics in Mammalian Cells Elucidated with Quantitative Coherent Anti-Stokes Raman Scattering Microspectroscopyen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0093401en_US
dc.identifier.journalPLOS ONEen_US
dc.citation.volume9en_US
dc.citation.issue4en_US
dc.citation.epageen_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000334159800031-
dc.citation.woscount2-
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