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dc.contributor.authorYang, Chi-Dungen_US
dc.contributor.authorChen, Yen-Huaen_US
dc.contributor.authorHuang, Hsi-Yuanen_US
dc.contributor.authorHuang, Hsien-Daen_US
dc.contributor.authorTseng, Ching-Pingen_US
dc.date.accessioned2014-12-08T15:36:21Z-
dc.date.available2014-12-08T15:36:21Z-
dc.date.issued2014-06-01en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.urihttp://dx.doi.org/10.1111/mmi.12614en_US
dc.identifier.urihttp://hdl.handle.net/11536/24683-
dc.description.abstractThe CRISPR/Cas system is an important aspect in bacterial immunology. The anti-phage activity of the CRISPR system has been established using synthetic CRISPR spacers, but in vivo studies of endogenous CRISPR spacers are relatively scarce. Here, we showed that bacteriophage P1 titre in Escherichia coli decreased in the glucose-containing medium compared with that in the absence of glucose. This glucose effect of E. coli against phage P1 infection disappeared in cse3 deletion mutants. The effect on the susceptibility to phage P1 was associated with cAMP receptor protein (CRP)-mediated repression of cas genes transcription and crRNA maturation. Analysis of the regulatory element in the cse1 promoter region revealed a novel CRP binding site, which overlapped with a LeuO binding site. Furthermore, the limited sequence identity between endogenous spacers and the phage P1 genome was necessary and sufficient for CRISPR-mediated repression of phage P1 replication. Trans-expression of the third and seventh spacers in the CRISPR I region or third and sixth spacers in the CRISPR II region effectively reduced phage P1 titres in the CRISPR deletion mutants. These results demonstrate a novel regulatory mechanism for cas repression by CRP and provide evidence that endogenous spacers can repress phage P1 replication in E. coli.en_US
dc.language.isoen_USen_US
dc.titleCRP represses the CRISPR/Cas system in Escherichia coli: evidence that endogenous CRISPR spacers impede phage P1 replicationen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/mmi.12614en_US
dc.identifier.journalMOLECULAR MICROBIOLOGYen_US
dc.citation.volume92en_US
dc.citation.issue5en_US
dc.citation.spage1072en_US
dc.citation.epage1091en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.identifier.wosnumberWOS:000337560500013-
dc.citation.woscount0-
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