標題: | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
作者: | Miyaoka, Rimi Hosokawa, Masahito Ando, Masahiro Mori, Tetsushi Hamaguchi, Hiro-o Takeyama, Haruko 應用化學系 應用化學系分子科學碩博班 Department of Applied Chemistry Institute of Molecular science |
關鍵字: | Raman microspectroscopy;in situ detection;antibiotics;secondary metabolites;actinomycetes |
公開日期: | 1-五月-2014 |
摘要: | The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes-Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling. |
URI: | http://dx.doi.org/10.3390/md12052827 http://hdl.handle.net/11536/24734 |
ISSN: | 1660-3397 |
DOI: | 10.3390/md12052827 |
期刊: | MARINE DRUGS |
Volume: | 12 |
Issue: | 5 |
起始頁: | 2827 |
結束頁: | 2839 |
顯示於類別: | 期刊論文 |