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dc.contributor.authorHsieh, Yin-Chengen_US
dc.contributor.authorChiu, Hsi-Hoen_US
dc.contributor.authorHuang, Yen-Chiehen_US
dc.contributor.authorFun, Hoong-Kunen_US
dc.contributor.authorLu, Chia-Yuen_US
dc.contributor.authorLi, Yaw-Kuenen_US
dc.contributor.authorChen, Chun-Jungen_US
dc.date.accessioned2014-12-08T15:36:49Z-
dc.date.available2014-12-08T15:36:49Z-
dc.date.issued2014-09-01en_US
dc.identifier.issn1744-3091en_US
dc.identifier.urihttp://dx.doi.org/10.1107/S2053230X14014629en_US
dc.identifier.urihttp://hdl.handle.net/11536/25200-
dc.description.abstractGlycosyltransferases (GTs), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. Glycosyltransferase-1 from Bacillus cereus (BcGT-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (UDP-glucose) as the sugar donor. BcGT-1 (molecular mass 45.5 kDa) has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction of BcGT-1 crystals to 2.10 angstrom resolution, the crystal belonged to space group P1, with unit-cell parameters a = 54.56, b = 84.81, c = 100.12 A, alpha = 78.36, beta = 84.66, gamma = 84.84 degrees. Preliminary analysis indicates the presence of four BcGT-1 molecules in the asymmetric unit with a solvent content of 50.27%.en_US
dc.language.isoen_USen_US
dc.titlePurification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereusen_US
dc.typeArticleen_US
dc.identifier.doi10.1107/S2053230X14014629en_US
dc.identifier.journalACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONSen_US
dc.citation.volume70en_US
dc.citation.issueen_US
dc.citation.spage1228en_US
dc.citation.epage1231en_US
dc.contributor.department交大名義發表zh_TW
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentNational Chiao Tung Universityen_US
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000341818600020-
dc.citation.woscount0-
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