Title: A pH-responsive amphiphilic chitosan-pyranine core-shell nanoparticle for controlled drug delivery, imaging and intracellular pH measurement
Authors: Chou, Hao-Syun
Hsiao, Meng-Hsuan
Hung, Wei-Yang
Yen, Tin-Yo
Lin, Hui-Yi
Liu, Dean-Mo
交大名義發表
材料科學與工程學系
National Chiao Tung University
Department of Materials Science and Engineering
Issue Date: 2014
Abstract: A pH-responsive multifunctional core-shell nanoparticle, named CHC-PY nanoparticle, was successfully synthesized through electrostatic interaction of a thin shell of fluorescent pyranine dye (PY) with amphiphilic carboxymethylhexanoyl chitosan (CHC) nanoparticles. Upon encapsulating an anticancer drug, camptothecin (CPT), the CHC-PY nanoparticles exhibited an excellent drug loading efficiency (>95%). The resulting CPT-loaded CHC-PY nanoparticles also exhibited efficient cell internalization and good pH-responsive behavior. After being internalized (via efficient endocytosis pathway), the presence of fluorescent PY shell showed a pH-dependent emission characteristic which allowed the internalized CHC-PY nanoparticles acting as an indicator to distinguish the acidic microenvironment of cancerous cells, compared with normal cells. The pH-sensitive PY shell also acted as a modulator to control the CPT release wherein a higher release rate was detected at lower pH value, which is essentially a potential therapeutic niche for anticancer purposes. This new type of CHC-PY core-shell nanoparticle provides multiple functionality, where a synergistic performance of nanotherapeutics, imaging and even diagnosis at a cellular resolution can be achieved simultaneously.
URI: http://hdl.handle.net/11536/25256
http://dx.doi.org/10.1039/c4tb01080a
ISSN: 2050-750X
DOI: 10.1039/c4tb01080a
Journal: JOURNAL OF MATERIALS CHEMISTRY B
Volume: 2
Issue: 38
Begin Page: 6580
End Page: 6589
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