完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chuang, Tsung-Hsien | en_US |
dc.contributor.author | Lai, Chao-Yang | en_US |
dc.contributor.author | Tseng, Ping-Hui | en_US |
dc.contributor.author | Yuan, Chiun-Jye | en_US |
dc.contributor.author | Hsu, Li-Chung | en_US |
dc.date.accessioned | 2014-12-08T15:36:58Z | - |
dc.date.available | 2014-12-08T15:36:58Z | - |
dc.date.issued | 2014-09-30 | en_US |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1371/journal.pone.0108808 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/25367 | - |
dc.description.abstract | CpG-oligodeoxynucleotides (CpG-ODN) are potent immune stimuli being developed for use as adjuvants in different species. Toll-like receptor 9 (TLR9) is the cellular receptor for CpG-ODN in mammalian cells. The CpG-ODN with 18-24 deoxynucleotides that are in current use for human and mouse cells, however, have low activity with rabbit TLR9. Using a cell-based activation assay, we developed a type of CpG-ODN containing a GACGTT or AACGTT motif in 12 phosphorothioate-modified deoxynucleotides with potent stimulatory activity for rabbit TLR9. The developed CpG-ODN have higher activities than other developed CpG-ODN in eliciting antigen-nonspecific immune responses in rabbit splenocytes. When mixed with an NJ85 peptide derived from rabbit hemorrhagic disease virus, they had potent activities to boost an antigen-specific T cell activation and antibody production in rabbits. Compared to Freund\'s adjuvant, the developed CpG-ODN are capable of boosting a potent and less toxic antibody response. The results of this study suggest that both the choice of CpG-motif and its length are important factors for CpG-ODN to effectively activate rabbit TLR9 mediated immune responses. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1371/journal.pone.0108808 | en_US |
dc.identifier.journal | PLOS ONE | en_US |
dc.citation.volume | 9 | en_US |
dc.citation.issue | 9 | en_US |
dc.citation.epage | en_US | |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000343671700167 | - |
dc.citation.woscount | 1 | - |
顯示於類別: | 期刊論文 |