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dc.contributor.authorHo, KCen_US
dc.contributor.authorTsai, PJen_US
dc.contributor.authorLin, YSen_US
dc.contributor.authorChen, YCen_US
dc.date.accessioned2014-12-08T15:37:10Z-
dc.date.available2014-12-08T15:37:10Z-
dc.date.issued2004-12-15en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac048688ben_US
dc.identifier.urihttp://hdl.handle.net/11536/25549-
dc.description.abstractIn this paper, we report a method for fabricating biofunctionalized nanoparticles by attaching human immunoglobulin (IgG) onto their surfaces through either electrostatic interactions or covalent binding. We found that these IgG-presenting nanoparticles can bind selectively to the cell walls of pathogens that contain IgG-binding sites based on the investigation of transmission electron microscopy images. Our results demonstrate that such Au-IgG nanoparticles may serve as useful nanoscale probes for exploring the interactions between IgG and pathogens. Furthermore, the IgG-presenting magnetic nanoparticles have been employed as effective affinity probes for selectively concentrating traces of target bacteria from sample solutions. The trapped bacteria were then characterized by using matrix-assisted laser desorption/ionization mass spectrometry. The lowest cell concentration we detected for both Staphylococcus saprophyticus and Staphylococcus aureus in aqueous sample solutions (0.5 mL) was similar to3 x 10(5) cfu/mL, while the detectable cell concentration for S. saprophyticus in a urine sample was similar to3 x 10(7) cfu/mL.en_US
dc.language.isoen_USen_US
dc.titleUsing biofunctionalized nanoparticles to probe pathogenic bacteriaen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac048688ben_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume76en_US
dc.citation.issue24en_US
dc.citation.spage7162en_US
dc.citation.epage7168en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000225781700002-
dc.citation.woscount102-
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