標題: Effects of alpha and beta recombinant FSH (Gonal-F, Puregon) and progesterone upon human endometrial cell proliferation in-vitro: a preliminary study
作者: Chang, Chi-Chen
Hsieh, Yao-Yuan
Hsu, Kung-Hao
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: Endometrium;gonadotrophin;Gonal-F;progesterone;Puregon
公開日期: 1-二月-2011
摘要: Endometrial proliferation or regeneration during menstrual cycle is regulated by sexual hormones. However, the effect of gonadotrophins on the endometrial cell growth remains obscure. Herein, we aimed to investigate the effects of r-FSH (Gonal-F, Puregon) and progesterone on the proliferation of human endometrial cells in-vitro. According as gonadotrophin concentrations, the follicular-phase endometrial cells were divided into six groups: (1) 0 (controls), (2) 1; (3) 10; (4) 100; (5) 1000; (6) 100,000 mu IU/ml. The cell countings with microscopy and cell proliferation kit assay were used to assess the endometrial cell proliferations. In Gonal-F groups, the cell absorptions (%) after 24/48 h culture were: (1) 100/100; (2) 103.8/102.3; (3) 104.8/102.8; (4) 102.3/101.3; (5) 96.3/94.2; (6) 86.8/84.3. In Puregon groups, the cell absorptions were: (1) 100/100; (2) 102.8/101.9; (3) 103/102.3; (4) 103.9/103.5; (5) 102.9/102.4; (6) 103.7/103.2 (non-different). In progesterone groups, the cell absorptions were: (1) 100/100; (2) 99.1/101.9; (3) 83.5/80.4; (4) 80.7/82.4. Higher dosage of Gonal-F (100,000 mu IU/ml) and progesterone (10, 100 mu g/ml) appeared the significant inhibition upon endometrium. We conclude that lower dosages of Gonal-F, Puregon, and progesterone appear the non-significant influence upon endometrium. Higher dosage of Gonal-F (10,000 mu IU/ml) and progesterone (10, 100 mu g/ml), but not Puregon, might interfere with the endometrial proliferation during follicular phase.</.
URI: http://dx.doi.org/10.3109/09513590.2010.495426
http://hdl.handle.net/11536/25801
ISSN: 0951-3590
DOI: 10.3109/09513590.2010.495426
期刊: GYNECOLOGICAL ENDOCRINOLOGY
Volume: 27
Issue: 2
起始頁: 110
結束頁: 116
顯示於類別:期刊論文