標題: Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells
作者: Okuno, Masanari
Hamaguchi, Hiro-o
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 15-Dec-2010
摘要: We have developed a multifocus confocal Raman microspectroscopic system for the fast multimode vibrational imaging of living cells. It consists of an inverted microscope equipped with a microlens array, a pinhole array, a fiber bundle, and a multichannel Raman spectrometer. Forty-eight Raman spectra from 48 foci under the microscope are simultaneously obtained by using multifocus excitation and image-compression techniques. The multifocus confocal configuration suppresses the background generated from the cover glass and the cell culturing medium so that high-contrast images are obtainable with a short accumulation time. The system enables us to obtain multimode (10 different vibrational modes) vibrational images of living cells in tens of seconds with only 1 mW laser power at one focal point. This image acquisition time is more than 10 times faster than that in conventional single-focus Raman microspectroscopy. (C) 2010 Optical Society of America
URI: http://dx.doi.org/10.1364/OL.35.004096
http://hdl.handle.net/11536/26225
ISSN: 0146-9592
DOI: 10.1364/OL.35.004096
期刊: OPTICS LETTERS
Volume: 35
Issue: 24
起始頁: 4096
結束頁: 4098
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