標題: | The treatment of propofol induced the TGF-beta 1 expression in human endothelial cells to suppress endocytosis activities of monocytes |
作者: | Li, Chi-Han Lee, Ru-Ping Lin, Yu-Ling Lin, Chen-Si Hsu, Bang-Gee Tseng, Feng-Jen Chen, Yu-Cheng Liao, Kuang-Wen Yang, Fwu-Lin 生物科技學系 分子醫學與生物工程研究所 Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering |
關鍵字: | Propofol;TGF-beta 1;Endothelial cells;Monocytes;Immunosuppression |
公開日期: | 1-十二月-2010 |
摘要: | Propofol anesthesia and sedation are known to downregulate the functions of many hematopoietic cells, such as macrophages and neutrophils, in vivo. However, the effects of propofol on secretion of the regulatory cytokine transforming growth factor beta 1 (TGF-beta 1) in vivo are unknown. In this study, the effects of propofol on TGF-beta 1 expression in human peripheral blood mononuclear cells, umbilical vein endothelial cells (HUVECs), lymphocytes (Jurkat) and monocytes (THP-1) were tested. Moreover, these sera were also tested for regulatory activity on monocyte endocytosis with or without treatment with the TGF-beta 1 pathway inhibitor SB431542. Propofol raised levels of both total and activated TGF-beta 1 in propofol-treated patient sera after surgical operations. Furthermore, propofol induced secretion of latent TGF-beta 1 in HUVEC cells and enhanced TGF-beta 1 activation in THP-1 and Jurkat cells in vitro. Additionally, sera from propofol-treated patients suppressed monocyte endocytosis ex vivo, an effect that was abrogated by the TGF-beta 1 pathway inhibitor SB431542. (C) 2010 Elsevier Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.cyto.2010.08.001 http://hdl.handle.net/11536/26308 |
ISSN: | 1043-4666 |
DOI: | 10.1016/j.cyto.2010.08.001 |
期刊: | CYTOKINE |
Volume: | 52 |
Issue: | 3 |
起始頁: | 203 |
結束頁: | 209 |
顯示於類別: | 期刊論文 |