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dc.contributor.authorKuo, CTen_US
dc.contributor.authorChen, HWen_US
dc.contributor.authorChen, JLen_US
dc.date.accessioned2014-12-08T15:38:57Z-
dc.date.available2014-12-08T15:38:57Z-
dc.date.issued2004-06-15en_US
dc.identifier.issn1570-0232en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jchromb.2003.12.012en_US
dc.identifier.urihttp://hdl.handle.net/11536/26666-
dc.description.abstractThis study developed an acid hydrolysis method instead of using enzyme extraction, equipped with column-switching system for the pretreatment of samples, in the determination of 1-hydroxypyrene in the urine from children and pyrene in airborne particulates. We collected both types of samples from areas near a petrochemical industry and rural areas as reference. Samples were first treated with acid hydrolysis and followed by solvent extraction prior to being injected into the separation system for the determination with high performance liquid chromatography and fluorescence. A column-switching system was on-line with a C-18 separation column to remove matrix interference and obtain a stable baseline of the chromatogram. The eluent used to separate the 1-hydroxypyrene was 60% (v/v) aqueous acetonitrile solution. A fluorescence detector was used to monitor 1-hydroxypyrene at lambda(ex) = 348 nm and lambda(em) = 388nm, and pyrene at lambda(ex) = 331 nm and lambda(em) = 390 nm. Both calibration graphs were linear with very good correlation coefficients (r > 0.999) and the detection limits were ca. 2 pg (5 ng/l). Results showed that there was a significant association between 1-hydroxypyrene levels in urine specimens and pyrene levels in airborne particulate samples (r = 0.68, P < 0.05). The average levels of pyrene in the particulates (0.18 versus 0.09 ng/m(3)) and of 1-hydroxypyrene in urine specimens (155.9 versus 110.2 ng/g creatinine) were higher for the petrochemical area than for the rural area. This method is stable and sensitive for measuring polycyclic aromatic hydrocarbons in environmental samples. (C) 2003 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subject1-hydroxypyreneen_US
dc.titleDetermination of 1-hydroxypyrene in children urine using column-switching liquid chromatography and fluorescence detectionen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jchromb.2003.12.012en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCESen_US
dc.citation.volume805en_US
dc.citation.issue2en_US
dc.citation.spage187en_US
dc.citation.epage193en_US
dc.contributor.department環境工程研究所zh_TW
dc.contributor.departmentInstitute of Environmental Engineeringen_US
dc.identifier.wosnumberWOS:000221573700001-
dc.citation.woscount21-
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