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dc.contributor.authorLin, YSen_US
dc.contributor.authorYang, CHen_US
dc.contributor.authorChen, YCen_US
dc.date.accessioned2014-12-08T15:39:51Z-
dc.date.available2014-12-08T15:39:51Z-
dc.date.issued2004en_US
dc.identifier.issn0951-4198en_US
dc.identifier.urihttp://hdl.handle.net/11536/27233-
dc.description.abstractA glass-chip-based sample preparation method for matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis of tryptic digests of proteins and intact cells is described. A MALDI matrix, 2,5-dihydroxybenzoic acid (2,5-DHB), was hybridized with sol-gels to generate a sol-gel-derived material. Taking advantage of the characteristics of sol-gels, the sol-gel-derived material readily adhered to the surface of a glass chip through covalent bonding. Only one step of sample preparation, deposition of the sample solution on the glass chip, was required before MALDI-MS analysis. Because 2,5-DHB was homogeneously dispersed on the sol-gel network structure, good spot-to-spot reproducibility was obtained in MALDI analysis using this approach and the analyte signals were uniform throughout the chip. The modified glass chips were robust and effective for at least 1 week. This glass-chip-based matrix preparation method provides a straightforward approach to developing techniques for analyzing the on-chip enzymatic digestion of proteins and intact cells of microorganisms. Cytochrome C and Escherichia coli were used as analytes to demonstrate the feasibility of this approach. The products of the on-chip enzymatic digests were identified through protein database searches. Copyright (C) 2004 John Wiley Sons, Ltd.en_US
dc.language.isoen_USen_US
dc.titleGlass-chip-based sample preparation and on-chip trypic digestion for matrix-assisted laser desorption/ionization mass spectrometric analysis using a sol-gel/2,5-dihydroxybenzoic acid hybrid matrixen_US
dc.typeArticleen_US
dc.identifier.journalRAPID COMMUNICATIONS IN MASS SPECTROMETRYen_US
dc.citation.volume18en_US
dc.citation.issue3en_US
dc.citation.spage313en_US
dc.citation.epage318en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000188695200012-
dc.citation.woscount13-
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