完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, YS | en_US |
dc.contributor.author | Yang, CH | en_US |
dc.contributor.author | Chen, YC | en_US |
dc.date.accessioned | 2014-12-08T15:39:51Z | - |
dc.date.available | 2014-12-08T15:39:51Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.issn | 0951-4198 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/27233 | - |
dc.description.abstract | A glass-chip-based sample preparation method for matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis of tryptic digests of proteins and intact cells is described. A MALDI matrix, 2,5-dihydroxybenzoic acid (2,5-DHB), was hybridized with sol-gels to generate a sol-gel-derived material. Taking advantage of the characteristics of sol-gels, the sol-gel-derived material readily adhered to the surface of a glass chip through covalent bonding. Only one step of sample preparation, deposition of the sample solution on the glass chip, was required before MALDI-MS analysis. Because 2,5-DHB was homogeneously dispersed on the sol-gel network structure, good spot-to-spot reproducibility was obtained in MALDI analysis using this approach and the analyte signals were uniform throughout the chip. The modified glass chips were robust and effective for at least 1 week. This glass-chip-based matrix preparation method provides a straightforward approach to developing techniques for analyzing the on-chip enzymatic digestion of proteins and intact cells of microorganisms. Cytochrome C and Escherichia coli were used as analytes to demonstrate the feasibility of this approach. The products of the on-chip enzymatic digests were identified through protein database searches. Copyright (C) 2004 John Wiley Sons, Ltd. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Glass-chip-based sample preparation and on-chip trypic digestion for matrix-assisted laser desorption/ionization mass spectrometric analysis using a sol-gel/2,5-dihydroxybenzoic acid hybrid matrix | en_US |
dc.type | Article | en_US |
dc.identifier.journal | RAPID COMMUNICATIONS IN MASS SPECTROMETRY | en_US |
dc.citation.volume | 18 | en_US |
dc.citation.issue | 3 | en_US |
dc.citation.spage | 313 | en_US |
dc.citation.epage | 318 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000188695200012 | - |
dc.citation.woscount | 13 | - |
顯示於類別: | 期刊論文 |