完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wu, HT | en_US |
dc.contributor.author | Chou, CK | en_US |
dc.contributor.author | Lin, CS | en_US |
dc.contributor.author | Huang, MC | en_US |
dc.date.accessioned | 2014-12-08T15:40:02Z | - |
dc.date.available | 2014-12-08T15:40:02Z | - |
dc.date.issued | 2003-12-01 | en_US |
dc.identifier.issn | 0936-6768 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1046/j.0936-6768.2003.00465.x | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/27351 | - |
dc.description.abstract | BALB/c mice are widely used in genetic, tumour and immunological studies. However, the mice demonstrate a lower reproduction rate, low fertility and small litters, because of their highly genetic homozygoisty. Based on in vitro fertilization (IVF), a routine technique for biomedical studies, it is worth to evaluate the effects to BALB/c mice on IVF efficiency. In order to test the genetic factor affecting the IVF efficiency of BALB/c, four reciprocal IVF tests of BALB/cByJ and FVB/NCrl mice were performed. The results showed that the average fertility of IVF sponsored by FVB/NCrl spermatozoa was 69.6%, but only 12.1% was obtained from BALB/cByJ strain. Effect of glucose contained in the culture medium to the IVF efficiency of BALB/cByJ was also evaluated. The results showed that the fertility of BALB/cByJ spermatozoa incubated with 0, 2.7, 5.5, 11.1 and 22.2 mM of glucose in the TYH medium were 6.8, 9.9, 13.9, 32.7 and 22.2%, respectively. It is showed that IVF efficiency of BALB/cByJ spermatozoa could be improved depending on the concentration of glucose in the IVF medium. According to the results, it is beleived that lower IVF of BALB/cByJ mice might be due to the genetic defect in spermatozoa and increasing glucose in the IVF medium which significantly affect the IVF efficiency of BALB/cByl via activating the spermatozoa. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Effects of glucose concentration on in vitro fertilization in BALB/c mice | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1046/j.0936-6768.2003.00465.x | en_US |
dc.identifier.journal | REPRODUCTION IN DOMESTIC ANIMALS | en_US |
dc.citation.volume | 38 | en_US |
dc.citation.issue | 6 | en_US |
dc.citation.spage | 470 | en_US |
dc.citation.epage | 474 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000186561700009 | - |
dc.citation.woscount | 6 | - |
顯示於類別: | 期刊論文 |