標題: | CMOS chip as luminescent sensor for biochemical reactions |
作者: | Lu, U Hu, BCP Shih, YC Yang, YS Wu, CY Yuan, CJ Ker, MD Wu, TK Li, YK Hsieh, YZ Hsu, WY Lin, CT 機械工程學系 生物科技學系 應用化學系 電子工程學系及電子研究所 Department of Mechanical Engineering Department of Biological Science and Technology Department of Applied Chemistry Department of Electronics Engineering and Institute of Electronics |
公開日期: | 1-六月-2003 |
摘要: | We describe a novel biochemical sensing method and its potential new biosensing applications. A light-sensitive complementary metal oxide semiconductor (CMOS) chip prepared through a standard 0.5-mum CMOS process was developed for measuring biochemical reactions. A light producing enzymatic reaction catalyzed by horseradish peroxidase (HRP) was designed as a platform reaction to determine the concentration of hydrogen. peroxide (H2O2) by the CMOS chip with a standard semiconductor parameter analyzer (HP4145). The kinetics of enzymatic reaction were determined and compared with a standard and sophisticated fluorometer (Hitachi F-4500) in a biochemical laboratory. Similar results were obtained by both instruments. Using glucose oxidase as an example, we further demonstrated that the HRP platform can be used to determine other H2O2 producing reactions with the CMOS system. The result points to an important application of the CMOS chip in biological measurements and in diagnosis of various health factors. |
URI: | http://dx.doi.org/10.1109/JSEN.2003.814653 http://hdl.handle.net/11536/27822 |
ISSN: | 1530-437X |
DOI: | 10.1109/JSEN.2003.814653 |
期刊: | IEEE SENSORS JOURNAL |
Volume: | 3 |
Issue: | 3 |
起始頁: | 310 |
結束頁: | 316 |
顯示於類別: | 期刊論文 |