完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wen, CM | en_US |
dc.contributor.author | Tseng, CS | en_US |
dc.contributor.author | Cheng, CY | en_US |
dc.contributor.author | Li, YK | en_US |
dc.date.accessioned | 2014-12-08T15:42:20Z | - |
dc.date.available | 2014-12-08T15:42:20Z | - |
dc.date.issued | 2002-06-01 | en_US |
dc.identifier.issn | 0885-4513 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1042/0885-4513:0350213 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/28751 | - |
dc.description.abstract | A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to > 90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzyme (ChiNCTU2) is a monomeric protein with an estimated molecular mass of 36.5 kDa and a pI of 6.3. It is thermally stable at 60 degreesC and pH 6-8 for more than 3 h. The optimal activity is in the range of 50-60 degreesC at pH 7.0. Chitobiose is the predominant product throughout the enzymic hydrolysis of the colloidal chitin, indicating that the purified chitinase is an exo-chitinase. Chito-oligosaccharides [with degree of polymerization (DP) values of 4-6] are good substrates of the purified enzyme, whereas a DP3 oligomer was slowly hydrolysed to form DP1 and DP2 sugars. The first 15 N-terminal amino acids of the enzyme were determined to be ANNLGSKLLVGYWHN, which is highly homologous to that of ChiA from Bacillus cereus. A PCR cloning technique was employed to obtain the corresponding gene from Bacillus NCTU2. The gene sequence was determined to be 1080 bp, encoding a polypeptide of 360 amino acids with the first 27 amino acids as the signal peptide. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Bacillus cereus | en_US |
dc.subject | chitin | en_US |
dc.subject | chito-oligosaccharide | en_US |
dc.title | Purification, characterization and cloning of a chitinase from Bacillus sp NCTU2 | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1042/0885-4513:0350213 | en_US |
dc.identifier.journal | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | en_US |
dc.citation.volume | 35 | en_US |
dc.citation.issue | en_US | |
dc.citation.spage | 213 | en_US |
dc.citation.epage | 219 | en_US |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000176640700008 | - |
dc.citation.woscount | 64 | - |
顯示於類別: | 期刊論文 |