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dc.contributor.authorPan, IHen_US
dc.contributor.authorLi, YKen_US
dc.date.accessioned2014-12-08T15:43:56Z-
dc.date.available2014-12-08T15:43:56Z-
dc.date.issued2001-04-15en_US
dc.identifier.issn0378-4347en_US
dc.identifier.urihttp://dx.doi.org/10.1016/S0378-4347(00)00604-6en_US
dc.identifier.urihttp://hdl.handle.net/11536/29706-
dc.description.abstractA rapid process for purification of an extracellular beta -xylosidase with high purity was developed. The manipulation involved the precipitation of protein from culture medium and the extraction of enzyme from the resuspended elude protein solution by an aqueous-two phase separation. A linear random copolymer, PE62, with 20% ethylene oxide and 80% propylene oxide was employed in both stages of the purification. The enzyme was precipitated effectively by using 10% (w/v) PE62 and 5% (w/v) Na2HPO4. The aqueous two-phase extraction was performed with PE62 (10%)-NaH2PO4 (15%) as phase-forming reagent. SDS-PAGE analysis revealed that the purified enzyme is near homogeneity. The yield is about 100% with a purification factor of 8.8-fold. The whole process can be completed within an hour without any column chromatography. (C) 2001 Elsevier Science B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectaqueous two-phase extractionen_US
dc.subjectenzymesen_US
dc.subjectbeta-xylosidaseen_US
dc.titleRapid process for purification of an extracellular beta-xylosidase by aqueous two-phase extractionen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S0378-4347(00)00604-6en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY Ben_US
dc.citation.volume754en_US
dc.citation.issue1en_US
dc.citation.spage179en_US
dc.citation.epage184en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000167900900021-
dc.citation.woscount11-
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