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dc.contributor.authorPan, IHen_US
dc.contributor.authorYao, HJen_US
dc.contributor.authorLi, YKen_US
dc.date.accessioned2014-12-08T15:44:15Z-
dc.date.available2014-12-08T15:44:15Z-
dc.date.issued2001-02-01en_US
dc.identifier.issn0141-0229en_US
dc.identifier.urihttp://dx.doi.org/10.1016/S0141-0229(00)00291-Xen_US
dc.identifier.urihttp://hdl.handle.net/11536/29878-
dc.description.abstractEffective extraction of protein from bulk medium is an important technique in bioresearch. In the present study, we describe an extracellular beta -xylosidase from the fermentation supernatant of Trichoderma koningii G-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. This two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (PEG) and sodium dihydrogenphosphate (NaH2PO4) in aqueous solution. beta -Xylosidase was recovered with high yield and high concentration in the bottom salt-rich phase when 25% (w/v) PEG 1500 and 20-25% (w/v) NaH2PO4 were applied. Based on a 1-liter scale extraction, the purity of the enzyme was enhanced at least 33-fold. The total activity increased 422% in comparison with that in the untreated filtrate. The effectiveness and simplicity may make this technique potentially useful in various applications. The transxylosylation activity of the enzyme purified by this technique was also investigated. (C) 2001 Elsevier Science Inc. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectbeta-xylosidaseen_US
dc.subjectaqueous two-phase systemen_US
dc.subjectpurificationen_US
dc.subjectpartitioningen_US
dc.titleEffective extraction and purification of beta-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioningen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/S0141-0229(00)00291-Xen_US
dc.identifier.journalENZYME AND MICROBIAL TECHNOLOGYen_US
dc.citation.volume28en_US
dc.citation.issue2-3en_US
dc.citation.spage196en_US
dc.citation.epage201en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000166621000010-
dc.citation.woscount17-
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