標題: Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization
作者: Lai, Yin-Hung
Wang, Chia-Chen
Lin, Sheng-Hsian
Lee, Yuan Tseh
Wang, Yi-Sheng
交大名義發表
National Chiao Tung University
公開日期: 4-十一月-2010
摘要: This work demonstrates a quantitative interpretation of ion desorption in matrix-assisted laser desorption/ionization (MALDI). The theoretical modeling incorporates transition state theory for the desorption of surface ions, assuming chemical and thermal equilibrium in the solid state prior to desorption. It is distinct from conventional models that assume chemical equilibrium in the gas phase. This solid-state thermodynamic interpretation was used to examine the desorption of pure 2,4,6-trihydroxyacetophenone (THAP) and of angiotensin I mixed with THAP. It successfully described the changes in ion yield with the effective temperature under various laser fluence and initial temperature conditions. The analysis also revealed the key role played by ion concentration in the modeling to provide the best fit of the model to observations. On the other hand, divergence of the ion beam with laser fluence was examined using an imaging detection method, and the signal saturation normally seen at high fluence was appropriately reduced by ion focusing. Simplified but deceptive theoretical interpretations were obtained when the analysis was conducted without adequate calibration of the instrument bias.
URI: http://dx.doi.org/10.1021/jp104250g
http://hdl.handle.net/11536/31945
ISSN: 1520-6106
DOI: 10.1021/jp104250g
期刊: JOURNAL OF PHYSICAL CHEMISTRY B
Volume: 114
Issue: 43
起始頁: 13847
結束頁: 13852
顯示於類別:期刊論文


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