標題: A novel role of CPEB3 in regulating EGFR gene transcription via association with Stat5b in neurons
作者: Peng, Shu-Chun
Lai, Yen-Ting
Huang, Hsi-Yuan
Huang, Hsien-Da
Huang, Yu-Shuian
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
公開日期: 1-Nov-2010
摘要: CPEB3 is a sequence-specific RNA-binding protein and represses translation of its target mRNAs in neurons. Here, we have identified a novel function of CPEB3 as to interact with Stat5b and inhibit its transcription activity in the nucleus without disrupting dimerization, DNA binding and nuclear localization of Stat5b. Moreover, CPEB3 is a nucleocytoplasm-shuttling protein with predominant residence in the cytoplasm; whereas activation of NMDA receptors accumulates CPEB3 in the nucleus. Using the knockdown approach, we have found the receptor tyrosine kinase, EGFR, is a target gene transcriptionally activated by Stat5b and downregulated by CPEB3 in neurons. The increased EGFR expression in CPEB3 knockdown neurons, when stimulated with EGF, alters the kinetics of downstream signaling. Taken together, CPEB3 has a novel function in the nucleus as to suppress Stat5b-dependent EGFR gene transcription. Consequently, EGFR signaling is negatively regulated by CPEB3 in neurons.
URI: http://dx.doi.org/10.1093/nar/gkq634
http://hdl.handle.net/11536/32016
ISSN: 0305-1048
DOI: 10.1093/nar/gkq634
期刊: NUCLEIC ACIDS RESEARCH
Volume: 38
Issue: 21
起始頁: 7446
結束頁: 7457
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