Inactivation of E. coli and B. subtilis by a parallel-plate dielectric barrier discharge jet

Abstract

Inactivation of Escherichia coli and Bacillus subtilis using the jet region of a parallel-plate air dielectric barrier discharge (DBD) driven by a distorted sinusoidal voltage power supply under atmospheric-pressure condition is reported. Various types of working gases, including pure N(2), pure O(2), and compressed air, were tested. Discharges were characterized electrically by measuring the discharge current, applied voltage and absorbed power, and optically by measuring the optical emission spectra in the UV-visible range and absorption spectra in the IR range. Results of survival rate show that both E. coil and B. subtilis cells (up to 10(7) CFU/mL) can be effectively inactivated using less than 18 passes (1.8 s of residence time in total) of exposure to the post-discharge jet region of compressed air and oxygen discharges at different treating distances in the range of 4-20 mm. Among these, the compressed-air discharge performs the best in inactivating both two types of the bacterial cells, because of the abundant ozone generation and probably through the assistance of nitrous oxide generated in the discharge. In summary, the post-discharge jet region of an oxygen-contained parallel-plate DBD driven by a distorted sinusoidal voltage power driven is very effective in inactivating both E. coli and B. subtilis cells under the present test conditions. Crown Copyright (c) 2010 Published by Elsevier B.V. All rights reserved.