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dc.contributor.authorChuang, Yao-Chenen_US
dc.contributor.authorLi, Jung-Chunen_US
dc.contributor.authorChen, Sz-Hauen_US
dc.contributor.authorLiu, Ting-Yuen_US
dc.contributor.authorKuo, Ching-Hanen_US
dc.contributor.authorHuang, Wei-Tingen_US
dc.contributor.authorLin, Chih-Shengen_US
dc.date.accessioned2014-12-08T15:48:40Z-
dc.date.available2014-12-08T15:48:40Z-
dc.date.issued2010-08-01en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.biomaterials.2010.04.026en_US
dc.identifier.urihttp://hdl.handle.net/11536/32368-
dc.description.abstractThe surface plasmon resonance (SPR) wavelength of colloidal gold nanoparticles (AuNPs) can vary when the AuNPs aggregate, have different sizes or shapes, or are modified with chemical molecules. In this study, an optical biosensing platform for a proteinase activity assay was established based on the SPR property of AuNPs. The 13-nm AuNPs were modified with gelatin (AuNPs-gelatin) as a proteinase substrate and subsequently modified with 6-mercaptohexan-1-ol (MCH) (AuNPs/MCH-gelatin). After proteinase (trypsin or gelatinase) digestion, the AuNPs lose shelter, and MCH increases the attractive force between the modified AuNPs. Therefore, the AuNPs gradually move closer to each other, resulting in AuNPs aggregation. The AuNPs aggregation can be monitored by the red shift of surface plasmon absorption and a visible color change of the AuNPs is from red to blue. Such a color change can be observed with the naked eye. For detection, the absorption ratio, A(625)/A(525), of the reacted AuNPs solution can be used to estimate quantitatively the proteinase activity. A linear correlation has been established with trypsin activity at concentrations from 1.25 x 10(-1) to 1.25 x 10(2) U and matrix metalloproteinase-2 activity at concentrations from 50 ng/mL to 600 ng/mL. (C) 2010 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subject6-Mercaptohexan-1-olen_US
dc.subjectGold nanoparticlesen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectOptical biosensoren_US
dc.subjectProteinaseen_US
dc.titleAn optical biosensing platform for proteinase activity using gold nanoparticlesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.biomaterials.2010.04.026en_US
dc.identifier.journalBIOMATERIALSen_US
dc.citation.volume31en_US
dc.citation.issue23en_US
dc.citation.spage6087en_US
dc.citation.epage6095en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000279719600020-
dc.citation.woscount32-
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