以電氣紡絲法製備引導骨頭再生膜之評估

Abstract

此研究利用電氣紡絲加工技術，將聚乳酸與碳酸鈣粉末進行混合射出加工，藉此提高可降解性之聚乳酸的剛性和韌性，尋求出對引導骨頭再生膜有最好的機械強度。 實驗過程中，以田口式實驗規劃法進行實驗組數簡化設計，以添加不同濃度與不同結晶相之碳酸鈣，藉由實驗結果探討不同結晶結構之碳酸鈣對於引導骨頭再生膜抗拉強度之影響。並由統計演算法則分析控制因子：操作電壓、溶液濃度、工作距離、幫浦流速等與引導骨頭再生膜抗拉強度之相關性。 拉伸測試結果顯示，添加霰石粉末之薄膜抗拉強度皆比添加方解石粉末之薄膜抗拉強度相對較高。兩者都具有良好的生物相容性，且在細胞貼附觀察中有明顯增生情形。

The purpose of this study was to investigate guided bone regeneration (GBR) membranes fabricated by Poly-L-lactic Acid (PLLA)/CaCO3 composite fibers and to discuss mechanical strength of GBR membranes. The composite fibers were successfully fabricated by electrospinning method. The experiments were arranged according to Taguchi method. Various percentages of calcium carbonates were added to the PLLA to compare its effects on the electrospinning. The calcium carbonates selected include commercial CaCO3 and Coral which is structurally different from the commercial CaCO3. The morphology of polymer fibers is influenced by various processing parameters such as, (1) viscosity of polymer solution determined by polymer concentration and additives, (2) applied voltage, (3) feeding rate of polymer solution, (4) distance between needle tip and collector. It is found that the tensile strength of GBR membrane composed of coral is far better than GBR membrane composed of commercial calcium carbonate. Besides, both GBR membranes have good cell attachment, the potential for the membranes to be used on human being is very strong.