以電生理訊號量測介電濕潤微液滴晶片之脂質雙層膜形成與離子通道嵌合

Abstract

離子通道的正常運作對於人體的健康佔著重要的地位，異常的離 子通道之相關疾病，包括：糖尿病、囊狀纖維化、精神分裂症等。電 生理量測技術為一種了解調控人體離子動態之離子通道的方法。而離 子通道之活性的展現，則是必須仰賴脂質雙層膜平台。本研究目的即 在於，藉由電生理訊號量測建構在介電濕潤晶片的脂質雙層膜之電容 值，確定雙層膜的存在；進一步觀察alpha-hemolysin 於脂質雙層膜 上的運作情形。經過運算所得的脂質雙層膜比電容值為0.5 μF/cm2；與雙層膜之理論值相當，且觀測alpha-hemolysin 離子電流呈現階梯狀訊號，即表示離子通道隨時間逐一鑲嵌於脂質雙層膜中，證明脂質雙層膜存在。由實驗結果得知單一alpha-hemolysin 導電率約為0.75nS，與文獻相符，並進而得知脂質雙層膜上的離子通道數。

Ion channels play an important role in human health. The malfunction of ion channels leads to diseases such as diabetes, cystic fibrosis, and schizophrenia. Electrophysiological measurement is one of the techniques to inspect ionic channels electrically, while stable bilayer lipid membranes are essential for active ion channels. In this research, we demonstrated a bilayer lipid membrane platform employed by electrowetting, allowing electrical recording to be conducted in a precisely controlled environment. The capacitance of bilayer lipid membrane was measured as 0.5 μF/cm2, indicating its bilayer structure. Ion channels, alpha-hemolysin (α-HL), were then incorporated into the formed bilayer lipid membrane. The recorded electrophysiological signal showed quantized ion current. The measured conductance of each channel was about 0.75 nS, concurring with previous studies.