開發可迴復性之流感病毒核酸檢測

Abstract

流感病毒在全球爆發以來造成公共衛生的危害，並生成新的流感毒株，可能導致毀滅性的大流行。因此，發展一個可靠的流感診斷方法對於臨床檢驗和近一步的治療是必要的。在此研究中，我們提出了一個新穎的核酸回復系統，其具有簡單，可逆，廣泛及生物相容之特性。核酸回復系統可藉由螢光檢測的方式探討液相懸浮和固相間核酸與核酸之雜合反應。雜合效率是具有濃度依賴性的，並且透過目標核酸和回復核酸濃度的增加，可以更輕易偵測反應的進行。我們的最終目標是將核酸回復技術與多晶矽奈米線場效電晶體整合，並作為生物感測之應用。初步結果表示，不論是在空氣中或水溶液的環境下，奈米線感測器已被證實具有良好的元件特性。未修飾及APTES修飾的奈米線場效電晶體可被視為是酸鹼值感測器，其具有極佳的性能，可即時檢測pH值變化。不久的將來，在生物醫學之應用上，多晶矽奈米線場效電晶體將作為檢驗核酸回復系統之平台。

The global outbreak of influenza virus infection causes a public health threat and generates periodically new strains of influenza that may result in devastating pandemic. Therefore, the development of a reliable influenza diagnostic approach is necessary for clinical inspection and advanced treatment. In this study, we proposed a novel DNA recovery system which possesses simple, reversible, extensive and biocompatible properties. The DNA recovery system was established on liquid and solid phase reaction for DNA-DNA hybridization with fluorescence detection. The hybridization efficiency was concentration-dependent, and the reaction was easily detected through the increasing target and recovery DNA. The final goal is to integrate the DNA recovery technology with the poly silicon nanowire field-effect transistors (poly-SiNW FETs) for biosensing application. Preliminary results indicated that the NW sensors possessed excellent electrical characteristics in air and aqueous solution. The unmodified and APTES-modified NW FETs also provided great performance as pH sensors for real-time detection. In the near future, the DNA recovery system will be tested on the poly-SiNW FET for biomedical application.