阻抗式生醫感測元件於PC12 細胞貼附與分化之鑑別研究

Abstract

在細胞培養過程中，細胞的貼附(cell adhesion)與延展(cell spreading)是黏著型細胞的基本生長過程，其中細胞型態的改變在醫療上可以為其病理狀態提供重要資訊。而細胞形態的改變，傳統上是使用光學的方式來進行觀測，然而生醫感測器則提供了另一種量測方式。本論文利用細胞與基質電阻抗判斷技術(Electric Cell-substrate Impedance Sensing, ECIS)來紀錄細胞貼附與分化所造成的阻抗變化，同時透過光學顯微鏡的影像確認訊號變化是來自細胞之不同生長貼附狀態。利用電阻抗監測體外細胞培養時，可以看出細胞貼附、分化與電阻抗存在有清楚的相關性，本研究所使用的實驗晶片裝置在特定的實驗條件下，可鑑別細胞貼附與分化的情形，不需要額外的生化檢測。除此之外，在本論文中也運用微流道結構導引細胞至目標位置上，以提供未來電阻抗量測系統與微流道整合之基礎。

During the cell culture process, cell adhesion and cell spreading are fundamental processes of adherent cells. There are some properties of living cells are associated with cell adhesion and cell spreading processes. The traditional way to monitor cells’ morphological changes is to use optical methods; however, biosensors provide an alternative measurement method. This study utilized Electric Cell-substrate Impedance Sensing (ECIS) method to record cell attachment and differentiation of the impedance changes and at the same time, the image through an optical microscope to confirm the signal change is different from cell growth and attachment status. Using electrical impedance monitoring in vitro cell culture, we can clearly identify cell adhesion and differentiation without any additional biochemical tests. The second part of this study is the guidance of cells to the target position by use of microfluidic structures. Combine these two techniques provide the possibility of single-cell detection in very low concentration in future applications.