利用電紡絲技術製作藥物傳遞系統支架

Abstract

靜電紡絲技術(electrospinning)是製造高分子奈米纖維十分有效 的技術。本研究將其應用於製作包覆藥物之奈米纖維支架，利用奈米 絲的表面孔隙作為釋放藥物的基礎，於紡絲內部充填藥物；相較於傳 統的給藥技術，此種釋放藥物方式大大提高作用時間，而且質量輕， 傷口密合度高，能夠有效提高傷口修復程度。本研究採用單軸、同軸 兩種電紡絲製作技術，製成數種內含藥物之奈米纖維，並且連續收集 而形成不織布纖維膜，藉由改變環境以及操作參數製作最有利於作為 藥物釋放之紡絲纖維支架。 研究中使用PEO做為紡絲纖維支架，於內部添加CS+Neomycin， 並利用各式顯微鏡(OM、SEM、TEM)探究分析紡絲纖維之外觀形貌， 表面孔隙以及內部結構，同時利用X光繞射分析其材料的結晶方向 對藥物釋放之變化差異性。控制濕度參數製作紡絲並同時提高紡絲良 率以及藥物分布變異；最後以能量色散譜儀(EDS)做為藥物釋放檢測 基礎，進行釋放含量分析，研究在歷經長時間變化之下，經由紡絲內 部向紡絲外部釋放藥物之釋放變化。研究結果發現，同軸奈米纖維之 藥物釋放特性遠比單軸奈米纖維的釋放效果明顯增強許多，當結晶特 性改變時，釋放效果也同時隨之發生變化。

Electrospinning is a highly effective technology for producing polymer nanofibers. This study utilizes this technology to produce nanofibrous frames to envelop drugs. The surface pores of the nanofibers are used to release the drugs that are contained in the fibers. Compared to traditional drug delivery systems, this method greatly increases the action time. This method also offers the advantages of low mass and high wound closure, effectively facilitating wound recovery. This study utilizes uniaxial and coaxial electrospinning technologies to produce nanofibers containing a number of different drugs, then continuously collects the nanofibers to form non-woven fiber membranes. This study then modifies the environment and manipulates the parameters to produce the most suitable spun fiber frame for drug release. This study utilizes PEO as a spun fiber frame and adds CS+neomycin internally. Various microscopes (OM, SEM, and TEM) are used to examine the external morphology of the spun fibers, including the surface porosity and internal structure. X-ray diffraction is also used to analyze the effect of crystal orientation on drug release variations. Humidity parameters are controlled while producing the fibers, increasing fiber yield rates and drug distribution variance. Finally, EDS is used as a basis for drug release testing. Release volume analysis is used to study variations in the internal to external release of drugs based on changes over a significant length of time. This study reveals that coaxial nanofiber drug release characteristics are significantly more powerful than those of uniaxial nanofibers. Additionally, when the crystal characteristics change, the release effects also change.