電子顯微鏡立體體積資料重組

Abstract

電子顯微鏡是觀測微觀生物常使用到的儀器之一，在處理適合的觀測的細胞樣本時，生物體需經過切片、染色、固定等步驟；在切片取樣本的過程中，也許會造成影像擷取的位置上的誤差。且切片後的單層影像所看到的只是局部資訊，倘若能把所有影像堆疊起來，那麼整體影像所提供的資訊勢必較多。 為了能夠提供更多影像上的資訊，本篇論文藉由尋找電子顯微影像的特徵區塊，利用影像對位（Image Alignamet）的方法將影像對位至相對應位置，將多張影像堆疊起來進而提供多方面的參考，以彌補單張影像的不足。

An electron microscope is one of the common used instruments to observe microorganisms. The microorganisms need be slicing, dyeing and fixing before observation. In the slicing process, a sampling position may be shifted. Additionally, the single layer image of the sliced microorganisms (hereafter called “samples”) only can provide partial information. Stacking all respective images of the samples will help for obtaining more information than the single layer image provided. Therefore, in this research, the method of image alignment is achieved by finding patterns of the images of the samples and aligning the images of that with the patterns to form a three-dimension image. Accordingly, the three-dimension image of the microorganisms provides many faces information to overcome the defect of the single layer image.