大氣電漿表面改質於聚乳酸人工血管支架對細胞培養之影響

Abstract

本研究利用兩個階段的大氣電漿處理，對電氣紡絲製備而成的聚乳酸人工血管支架進行改質。從第一個階段以氮氣/氧氣混合電漿對血管支架做親水性的提升並造成材料表面斷鍵；隨後以第二階段的氮氣/氨氣電漿進行胺基的導入。以不同處理次數的參數組合，探討兩個階段電漿對於細胞培養之影響。 經過電漿處理後的血管支架表面，親水性有確實的提升，並且從顯微觀察纖維結構，發現表面粗糙度增加，親水性與粗糙度都是對於細胞貼附皆有所幫助。另外從細胞活性分析探討親水性和胺基在細胞培養過程中的角色，發現在第三天時細胞貼附的效果是由親水性所決定，但是較佳的親水性卻不一定可以獲得較多的細胞貼附；而胺基的效果必須到第七天才會顯現出來，這是由於胺基並非直接與細胞作結合或反應，而是經由吸附培養液中的特定糖蛋白種類，幫助細胞在培養過程中獲取養分，並且隨著胺基電漿處理次數的增加，細胞活性有愈提升的趨勢。 而較長時間的親水電漿處理造成較多的斷鍵空缺，提供足夠的糖蛋白附著，也因此儘管在一開始細胞貼附的效果不佳，但長時間的培養還是可以獲得較佳的成長與增殖現象。

A 2-step atmospheric plasma was adopted for treating the vascular scaffold prepared by electrospinning. At first step, we mix nitrogen/oxygen to generate plasma to promote the vascular scaffold surface hydrophilic property bond. Subsequently, the second step which uses nitrogen/ammonia plasma to graft amine group. The effect of cell culture on the vascular scaffold through 2-step plasma treatment in different parameter were compared. The roughness and hydrophilic property bond on the vascular scaffold surface increase through plasma treatment. Hydrophilic property bond and roughness affect cell culture. In addition, we found that cell attachment is decided on hydrophilic property bond on third day, but the better hydrophilic property bond doesn't mean that more cell attachment. The effect of amine group can only be observed on seventh day. The results indicated that amine group doesn’t interact with the cell, but absorb particular glycoproteins that provide nutrition in cell culture. And increasing the treatment times of amine group plasma processing, cell activity is improved.