標題: | Functional gold nanoclusters as antimicrobial agents, for antibiotic-resistant bacteria |
作者: | Chen, Wei-Yu Lin, Ju-Yu Chen, Wei-Jen Luo, Liyang Diau, Eirc Wei-Guang Chen, Yu-Chie 應用化學系 Department of Applied Chemistry |
關鍵字: | antibiotic-resistant bacteria;Au nanoclusters;drug delivery;fluorescence;lysozyme;microwave heating |
公開日期: | 1-七月-2010 |
摘要: | Aims: Our aim was to demonstrate that lysozyme-directed generation of gold nanoclusters (Au NCs) are potential antimicrobial agents for antibiotic-resistant bacteria and broad labeling agents for pathogenic bacteria. Materials & methods: Lysozyme is an enzyme that is capable of hydrolyzing the cell walls of bacteria. In this study, we demonstrated the generation of functional Au NCs by using lysozyme as the sequester and the reducing agent for Au precursors at 40 degrees C. In addition, to shorten the reaction time, the reaction was conducted under microwave irradiation within a short period of time for the first time. Results: The bioactivity of the lysozyme on the Au NCs was retained. Therefore, the as-prepared lysozyme Au NCs with desirable fluorescence feature were successfully employed to be broad-band labeling agents for pathogenic bacteria. Furthermore, we also demonstrated that the lysozyme Au NCs can be used to effectively inhibit the cell growth of notorious antibiotic-resistant bacteria, including pan-drug-resistant Acinetobacter baumannii and vancomycin-resistant Enterococcus faecalis. Conclusion: The potential of employing the lysozyme Au NCs for bacterial labeling and as antimicrobial agents is expected. |
URI: | http://dx.doi.org/10.2217/nnm.10.43 http://hdl.handle.net/11536/5176 |
ISSN: | 1743-5889 |
DOI: | 10.2217/nnm.10.43 |
期刊: | NANOMEDICINE |
Volume: | 5 |
Issue: | 5 |
起始頁: | 755 |
結束頁: | 764 |
顯示於類別: | 期刊論文 |