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dc.contributor.authorSun, Li-Yien_US
dc.contributor.authorHsieh, Dean-Kuoen_US
dc.contributor.authorLin, Po-Chengen_US
dc.contributor.authorChiu, Hsien-Taien_US
dc.contributor.authorChiou, Tzyy-Wenen_US
dc.date.accessioned2014-12-08T15:07:06Z-
dc.date.available2014-12-08T15:07:06Z-
dc.date.issued2010-04-01en_US
dc.identifier.issn0197-8462en_US
dc.identifier.urihttp://dx.doi.org/10.1002/bem.20550en_US
dc.identifier.urihttp://hdl.handle.net/11536/5570-
dc.description.abstractOsteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined the effect of pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and gene expression in bone marrow mesenchymal stem cells (BMMSCs) during osteogenic differentiation. Exposure of BMMISCs to PEMFs increased cell proliferation by 29.6% compared to untreated cells at day 1 of differentiation. Semi-quantitative RT-PCR indicated that PEMFs significantly altered temporal expression of osteogenesis-related genes, including a 2.7-fold increase in expression of the key osteogenesis regulatory gene cbfa1, compared to untreated controls. In addition, exposure to PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis. These results suggest that PEMFs enhance early cell proliferation in B M MSC-mediated osteogenesis, and accelerate the osteogenesis. Bioelectromatmetics 31:209-219, 2010. (C) 2009 Wiley-Liss, Inc.en_US
dc.language.isoen_USen_US
dc.subjectpulsed electromagnetic fieldsen_US
dc.subjectosteogenesisen_US
dc.subjectmesenchymal stem cellsen_US
dc.titlePulsed Electromagnetic Fields Accelerate Proliferation and Osteogenic Gene Expression in Human Bone Marrow Mesenchymal Stem Cells During Osteogenic Differentiationen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/bem.20550en_US
dc.identifier.journalBIOELECTROMAGNETICSen_US
dc.citation.volume31en_US
dc.citation.issue3en_US
dc.citation.spage209en_US
dc.citation.epage219en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000276052600005-
dc.citation.woscount19-
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