標題: | The cAMP Receptor-Like Protein CLP Is a Novel c-di-GMP Receptor Linking Cell-Cell Signaling to Virulence Gene Expression in Xanthomonas campestris |
作者: | Chin, Ko-Hsin Lee, Yen-Chung Tu, Zhi-Le Chen, Chih-Hua Tseng, Yi-Hsiung Yang, Jinn-Moon Ryan, Robert P. McCarthy, Yvonne Dow, J. Maxwell Wang, Andrew H. -J. Chou, Shan-Ho 生物資訊及系統生物研究所 Institude of Bioinformatics and Systems Biology |
關鍵字: | Xcc;pathogenicity;CRP;CLP;c-di-GMP receptor |
公開日期: | 26-Feb-2010 |
摘要: | Cyclic-di-GMP [bis-(3'-5')-cyclic diguanosine monophosphate] controls a wide range of functions in eubacteria, yet little is known about the underlying regulatory mechanisms. In the plant pathogen Xanthomonas campestris, expression of a subset of virulence genes is regulated by c-di-GMP and also by the CAP (catabolite activation protein)-like protein XcCLP, a global regulator in the CRP/FNR superfamily. Here, we report structural and functional insights into the interplay between XcCLP and c-di-GMP in regulation of gene expression. XcCLP bound target promoter DNA with submicromolar affinity in the absence of any ligand. This DNA-binding capability was abrogated by c-di-GMP, which bound to XcCLP with micromolar affinity. The crystal structure of XcCLP showed that the protein adopted an intrinsically active conformation for DNA binding. Alteration of residues of XcCLP implicated in c-di-GMP binding through modeling studies caused a substantial reduction in binding affinity for the nucleotide and rendered DNA binding by these variant proteins insensitive to inhibition by c-di-GMP. Together, these findings reveal the structural mechanism behind a novel class of c-di-GMP effector proteins in the CRP/FNR superfamily and indicate that XcCLP regulates bacterial virulence gene expression in a manner negatively controlled by the c-di-GMP concentrations. (C) 2009 Elsevier Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.jmb.2009.11.076 http://hdl.handle.net/11536/5820 |
ISSN: | 0022-2836 |
DOI: | 10.1016/j.jmb.2009.11.076 |
期刊: | JOURNAL OF MOLECULAR BIOLOGY |
Volume: | 396 |
Issue: | 3 |
起始頁: | 646 |
結束頁: | 662 |
Appears in Collections: | Articles |
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