標題: | Alteration of the Substrate's Prefolded Conformation and Cyclization Stereochemistry of Oxidosqualene-Lanosterol Cyclase of Saccharomyces cerevisiae by Substitution at Phenylalanine 699 |
作者: | Wu, Tung-Kung Chang, Cheng-Hsiang Wen, Hao-Yu Liu, Yuan-Ting Li, Wen-Hsuan Wang, Tsai-Ting Shie, Wen-Shiang 生物科技學系 Department of Biological Science and Technology |
公開日期: | 5-二月-2010 |
摘要: | The Saccharomyces cerevisiae ERG7(Phe699) mutants produced one chair-chair-chair (C-C-C) and two chair-boat-chair (C-B-C) truncated tricyclic compounds, one tetracyclic 17 alpha-exocyclic unrearranged intermediate, and two 17 beta-exocyclic truncated rearranged intermediates. These results provided direct evidence for the importance of the residue in affecting mechanistic transitions between C-B-C and C-C-C substrate conformation and between the 17 alpha- and 17 beta-exocyclic side chain stereochemistry as well as in stabilizing the 6 -6-5 tricyclic and the protosteryl C-17 cations. |
URI: | http://dx.doi.org/10.1021/ol902694y http://hdl.handle.net/11536/5850 |
ISSN: | 1523-7060 |
DOI: | 10.1021/ol902694y |
期刊: | ORGANIC LETTERS |
Volume: | 12 |
Issue: | 3 |
起始頁: | 500 |
結束頁: | 503 |
顯示於類別: | 期刊論文 |