標題: | Identification of the mokH Gene Encoding Transcription Factor for the Upregulation of Monacolin K Biosynthesis in Monascus pilosus |
作者: | Chen, Yi-Pei Yuan, Gwo-Fang Hsieh, Sung-Yuan Lin, Yu-Shan Wang, Wei-Yi Liaw, Li-Ling Tseng, Ching-Ping 生醫工程研究所 Institute of Biomedical Engineering |
關鍵字: | Monacolin K;Monascus pilosus;transcription factor;glyceraldehyde-3-phosphate dehydrogenase |
公開日期: | 13-一月-2010 |
摘要: | Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus. The monacolin K biosynthetic gene cluster, mokA-mokI, has been characterized in Monascus pilosus. The mokH gene encoding Zn(II)2Cys6 binuclear DNA binding protein is assumed to be an activator for monacolin K production. In this study, the mokH gene was cloned and driven by the glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter for overexpression in M. pilosus. The transformants containing an extra copy of the mokH gene were obtained and verified by PCR and Southern hybridization. The transcripts of mokH in the transformants were expressed significantly higher than those of the wild-type strain. The transformants were stably inherited through the next generation, as determined by observation of the enhanced green fluorescent protein (EGFP). The transformant T-mokH1 also showed a 1.7-fold higher production of monacolin K than the wild-type strain in a time course analysis. Analysis of the RT-PCR products demonstrated that the monacolin K biosynthetic genes in the transformant were expressed to a greater extent than those in the wildtype strain. These results indicated that mokH upregulated the transcription of monacolin K biosynthetic genes and increased monacolin K production. |
URI: | http://dx.doi.org/10.1021/jf903139x http://hdl.handle.net/11536/5976 |
ISSN: | 0021-8561 |
DOI: | 10.1021/jf903139x |
期刊: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
Volume: | 58 |
Issue: | 1 |
起始頁: | 287 |
結束頁: | 293 |
顯示於類別: | 期刊論文 |