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dc.contributor.authorChang, Yu-Jenen_US
dc.contributor.authorHwang, Shiaw-Minen_US
dc.contributor.authorTseng, Ching-Pingen_US
dc.contributor.authorCheng, Fu-Chouen_US
dc.contributor.authorHuang, Shih-Hungen_US
dc.contributor.authorHsu, Lee-Fengen_US
dc.contributor.authorHsu, Li-Wenen_US
dc.contributor.authorTsai, Ming-Songen_US
dc.date.accessioned2014-12-08T15:07:49Z-
dc.date.available2014-12-08T15:07:49Z-
dc.date.issued2010en_US
dc.identifier.issn1422-6405en_US
dc.identifier.urihttp://hdl.handle.net/11536/6155-
dc.identifier.urihttp://dx.doi.org/10.1159/000295774en_US
dc.description.abstractThe amniotic membrane has been clinically applied as a therapeutic material in wound covering and corneal surface reconstruction. Recently, mesenchymal stem cells (MSCs) have been isolated from the placenta, specifically from the amniotic membrane. However, the localization of MSCs in the amniotic membrane has not been determined. In this study, term placenta was collected, and we performed immunohistochemical staining techniques to identify and localize MSCs in the mesoderm of the amniotic membrane in situ with MSC antibodies, including CD90 and CD105. We further directly cultured and characterized MSCs from the amniotic membrane mesoderm (AMSCs). The AMSCs were easily isolated and represented a homogenous fibroblastic morphology at early passages. In addition to MSC surface markers, AMSCs expressed Sox2, Oct-4 and Nanog. AMSCs could be induced into osteocytes, adipocytes and chondrocytes in vitro and show immunosuppressive effects on T-cell proliferation. Under appropriate conditions, AMSCs could differentiate into neuronal-like cells, which were identified by neuronal-specific markers and their ability to secrete dopamine. This study reveals that AMSCs provide a promising source for stem cell studies and also extend the clinical potential of the amniotic membrane in the field of regenerative medicine. Copyright (C) 2010 S. Karger AG, Baselen_US
dc.language.isoen_USen_US
dc.subjectAmniotic mesodermen_US
dc.subjectDopamine-producing cellsen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectMultipotent progenitorsen_US
dc.subjectRegenerative medicineen_US
dc.titleIsolation of Mesenchymal Stem Cells with Neurogenic Potential from the Mesoderm of the Amniotic Membraneen_US
dc.typeArticleen_US
dc.identifier.doi10.1159/000295774en_US
dc.identifier.journalCELLS TISSUES ORGANSen_US
dc.citation.volume192en_US
dc.citation.issue2en_US
dc.citation.spage93en_US
dc.citation.epage105en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000280014600003-
dc.citation.woscount21-
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