固定細胞生產和回收溶葡萄球菌素之研究

Abstract

溶葡萄球菌素 ( lysostaphin ) 是由Staphylococcus simulans biovar staphylolyticus ATCC1362所產生，能破壞葡萄球菌 ( S. aureus ) 細胞壁五個glycine所組成□□的位置，具有殺菌 ( 金黃色葡 萄球菌 ) 的作用。溶葡萄球菌素基因已被選殖和定序出來，經基因重組 ( recombinant DNA ) 的菌株Escherichia coli JMply 6能分泌出溶葡萄 球菌素 ( lysostaphin )。本實驗利用細胞固定化的方法，收集培養達對 數生長期末期 ( late log phase ) 的E. coli JMply 6菌體，包埋在2% ( w/v ) 的褐藻膠 ( calcium alginate )中，利用此方式，進行批式 ( batch ) 和i Recombinant lysostaphin is a protein originally derived from Staphylococcus simulans biovar staphylolyticus ATCC1362, exerts a bactericidal effect upon S. aureus by enzymatically degrading the pentaglycine crosslinks of the cell wall. The gene of lysostaphin have been cloned and sequenced. Escherichia coli JMply 6 contains lysostaphin gene can produce the very protein. In this study we used the method of cell immobilization to produce lysostaphin. The E. coli JMply 6 cells harvested at the late log phase