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dc.contributor.authorLin, Jia-Jyunen_US
dc.contributor.authorChen, Jenn-Shingen_US
dc.contributor.authorHuang, Shih-Jeren_US
dc.contributor.authorKo, Jyun-Hanen_US
dc.contributor.authorWang, Yu-Mingen_US
dc.contributor.authorChen, Ting-Lungen_US
dc.contributor.authorWang, Li-Fangen_US
dc.date.accessioned2014-12-08T15:08:36Z-
dc.date.available2014-12-08T15:08:36Z-
dc.date.issued2009-10-01en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.biomaterials.2009.06.004en_US
dc.identifier.urihttp://hdl.handle.net/11536/6609-
dc.description.abstractSuperparamagnetic iron oxides possess specific magnetic properties in the presence of an external magnetic field, which make them an attractive platform as contrast agents for magnetic resonance imaging (MRI) and as carriers for drug delivery. In this study, we investigate the drug delivery and the MRI properties of folate-mediated water-soluble iron oxide incorporated into micelles. Pluronic (R) F127 (PF127), which can be self-assembled into micelles upon increasing concentration or raising temperatures, is used to decorate water-soluble polyacrylic acid-bound iron oxides (PAAIO) via a chemical reaction. Next, the hydrophobic dye Nile red is encapsulated into the hydrophobic poly(propylene oxide) compartment of PF127 as a model drug and as a fluorescent agent. Upon encapsulation, PAAIO retains its superparamagnetic characteristics, and thus can be used for MR imaging. A tumor-specific targeting ligand, folic acid (FA), is conjugated onto PF127-PAAIO to produce a multifunctional superparamagnetic iron oxide, FA-PF127-PAAIO. FA-PF127-PAAIO can be simultaneously applied as a diagnostic and therapeutic agent that specifically targets cancer cells that overexpress folate receptors in their cell membranes. PF127-PAAIO is used as a reference group. Based on FTIR and UV-vis absorbance spectra, the successful synthesis of PF127-PAAIO and FA-PF127-PAAIO is realized. The magnetic nanoparticle clusters of PF127-PAAIO and FA-PF127-PAAIO are visualized by transmission electron microscope (TEM). FA-PF127-PAAIO, together with a targeting ligand, displays a higher intracellular uptake into KB cells. This result is confirmed by laser confocal scanning microscopy (CLSM), flow cytometry, and atomic absorption spectroscopy (AAS) studies. The hysteresis curves, generated by using a superconducting quantum interference device (SQUID) magnetometer analysis, demonstrate that the magnetic nanoparticles are superparamagnetic with insignificant hysteresis. The MITT assay explains the negligible cell cytotoxicity of PF127-PAAIO and FA-PF127-PAAIO. In KB cells, the in vitro MRI study indicates the better T(2)-weighted images in FA-PF127-PAAIO than in PF127-PAAIO. (C) 2009 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectFe(3)O(4)en_US
dc.subjectPluronic F127en_US
dc.subjectFolic aciden_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectDiagnosisen_US
dc.subjectTherapyen_US
dc.titleFolic acid-Pluronic F127 magnetic nanoparticle clusters for combined targeting, diagnosis, and therapy applicationsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.biomaterials.2009.06.004en_US
dc.identifier.journalBIOMATERIALSen_US
dc.citation.volume30en_US
dc.citation.issue28en_US
dc.citation.spage5114en_US
dc.citation.epage5124en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000269459900029-
dc.citation.woscount97-
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