利用同步輻射X光合成以牛血清蛋白為基板之奈米金團簇並探討其尺寸與螢光行為演進之研究

Abstract

藉由強烈的X射線照射，我們成功合成以牛血清白蛋白（BSA）為基板的金奈米粒子。通過控制牛血清白蛋白（BSA）和金之間的摩爾濃度，我們可以調整金奈米粒子的大小從20nm到1.9nm。這些奈米粒子的大小將影響其光學特性，尤其針對於尺寸超小的奈米金團簇（<2 nm）。我們的研究結果表明，以牛血清白蛋白（BSA）為基板的奈米金團簇擁有光激螢光特性並有兩個主要的發射波長（635nm和710nm）。藉由變溫螢光光譜,我們更進一步確定了其中主要發射波長635奈米是來自於奈米金團簇核心本身的能帶結構。同時，我們也嘗試合成金銀的複合奈米團簇並測量其生物相容性。 以牛血清白蛋白（BSA）為基板的奈米金團簇，奈米金銀複合團簇和奈米銀團簇都表現出較高的螢光強度和良好的生物相容性，期望可適用於生物圖像應用和敏感的熒光傳感器。

Gold nanoparticles were synthesized by intense X-ray irradiation in the presence of bovine serum albumin (BSA). By control the molar concentration between BSA and gold, we can adjust the gold nanoparticles size from 20 nm to 1.9 nm. The influence of these nanoparticles size on the optical properties of these ultra-small gold nanoclusters (<2 nm) was investigated. Our results show that the gold nanoclusters capped with bovine serum albumin (BSA) produced photoluminescent with two major emission wavelength from gold nanoclusters (635 nm and 710 nm). We confer the source of emission wavelength at 635 nm is core of gold nanoclusters band gaps by temperature dependent fluorescence spectroscopy. Simultaneously, we also try to synthesize gold/silver complex nanoclusters and measure their biocompatibility. The BSA coated gold nanoclusters, gold/silver complex nanoclusters and silver nanoclusters exhibit higher fluorescent intensity and good biocompatibility suitable for applications in bio-image and sensitive fluorescence sensors.