與金奈米粒子共軛之自組裝超分子胜肽水膠生物材料之合成及分析

Abstract

在近年來，水膠複合物在生物材料上的應用發展備受矚目，例如生物分析、化學檢測、藥物釋放。將不同奈米結構的材料做新穎的結合，有助於多功能材料的發展。在本篇研究中，合成2-Naphthylacetic acid- L-Phenylalanine- L-Phenylalanine- L-Cysteine (NapFFC) 自組裝超分子水膠並與金奈米粒子做共軛結合，為一種新穎且具有潛力的複合材料。對於環境因素改變形成膠體的條件，例如濃度、酸鹼值、離子強度、溫度及反應時間，可以藉由視覺觀察得知。分析NapFFC與金奈米粒子之間的作用力，討論得知保護能力的來源，以及NapFFC和奈米粒子彼此為性質互補的材料。吾人觀察到因為自組裝分子所產生的纖維網狀並分析不同種類的螺旋結構。NapFFC在335奈米所釋放的螢光強度有助於判斷在60 μM以下的濃度，而在這範圍不會有自我消光的現象發生。利用金奈米粒子對於螢光有消光的作用，因其具備靈敏及準確的特性，連結在金奈米粒子分子數量亦能求得。在本篇未來研究展望中，吾人再使用一樣的螢光檢測方法，發現汞離子具有類似的消光特性。此外，在細胞存活率也表現出良好的生物相容性。本篇研究所做的分析結果對於日後在生物材料上的發展有所貢獻。

Recently, development of hydrogel nanocomposites offered tremendous scope for future biomaterial applications, including bioanalysis, chemical sensing, and drug delivery. Smart combinations of different nanostructured materials supported the development of multifunctional nanomaterials. In this study, synthesizing self-assembly peptide supramolecular hydrogel 2-Naphthylacetic acid- L-Phenylalanine- L-Phenylalanine- L-Cysteine (NapFFC) has been done and conjugated with gold nanoparticles (AuNPs) as a new and potential composite material. Finding stimuli-response properties for critical hydrogelation conditions such as concentration, pH, ionic strength, temperature, and incubation time were defined by visual. Further discussion for protection ability resulted from interaction between NapFFC and AuNPs was analyzed, and the results showed hydrogel and nanoparticles were complementary materials. Fibrous network and various kinds of helix structure resulted from self-assembly molecule blocks was observed and analyzed. Fluorescence intensity emitted at 335 nm was also helpful to obtain concentration of NapFFC under 60 μM within which self-quenching didn’t occur. Utilizing fluorescence quenching effect of AuNPs was quite sensitive and precise to measure the accurate amounts of molecules bound to the surface of AuNPs. This method was also used to detect quenching effectiveness of mercury ions in this study. Moreover, cell viability showed good biocompatibility. These analysis and results of composite material were attractive feature for exploitation in the field of biomaterials.