標題: Localization and characterization of VVA0331, a 489-kDa RTX-like protein, in Vibrio vulnificus YJ016
作者: Chou, Li-Fang
Peng, Hwei-Ling
Yang, Yu-Chung
Kuo, Min-Chieh
Chang, Hwan-You
生物科技學系
Department of Biological Science and Technology
關鍵字: Vibrio vulnificus;RTX;Agglutination;Outer membrane proteins
公開日期: 1-五月-2009
摘要: Vibrio vulnificus YJ016 contains three genes encoding proteins homologous to repeats-in-toxin proteins. One of these genes, vva0331, possesses a long open reading frame of 13,971 bp in length and resides on the small chromosome between two gene clusters encoding a type I secretion system and several regulatory proteins, respectively. Bioinformatic analysis revealed that VVA0331 consist of nineteen 87-amino acid repeats, two Arg-Gly-Asp motifs, four cysteine residues, an outer membrane protein domain, a polysaccharide-binding site and several motifs related to cell adhesions. These features are distinct from those of typical repeat-in-toxins and autotransporter adhesins. Real-time quantitative PCR analysis indicates that vva0331 gene expression is activated at 30A degrees C and regulated by iron. In addition, VVA0331 is present primarily in a secreted form as determined by cell fractionation assay and Western blot analysis. No significant difference in Hep2 cell adherence, cytotoxicity, and virulence was observed between the wild type and vva0331 mutant strains. In contrast, these strains exhibited apparently different outer membrane protein profiles, and antiserum raised against C-terminal region of VVA0331 reacted with an 85-kDa outer membrane protein of V. vulnificus YJ016.
URI: http://dx.doi.org/10.1007/s00203-009-0471-1
http://hdl.handle.net/11536/7303
ISSN: 0302-8933
DOI: 10.1007/s00203-009-0471-1
期刊: ARCHIVES OF MICROBIOLOGY
Volume: 191
Issue: 5
起始頁: 441
結束頁: 450
顯示於類別:期刊論文


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