標題: Iron Oxide/Niobium Oxide Core-Shell Magnetic Nanoparticle-Based Phosphopeptide Enrichment from Biological Samples for MALDI MS Analysis
作者: Lin, Hong-Yi
Chen, Wei-Yu
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
關鍵字: Magnetic Nanoparticles;Phosphopeptides;MALDI MS;Niobium Oxide;Microwave-Heating
公開日期: 1-四月-2009
摘要: The feasibility of using niobium oxide-coated magnetic nanoparticles (NPs) as affinity probes for selectively trapping phosphopeptides from peptide mixtures including tryptic digest of caseins, serum, and cell lysate was demonstrated in this study. Phosphopeptide enrichment was rapid when subjecting these systems to microwave heating for 1 min; the probe-target species, which were readily isolated through magnetic separation, were then analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Only signals for phosphopeptides were present in the resulting mass spectra. The detection limit for monophosphopeptide was as low as 5 fmol.
URI: http://dx.doi.org/10.1166/jbn.2009.1022
http://hdl.handle.net/11536/7398
ISSN: 1550-7033
DOI: 10.1166/jbn.2009.1022
期刊: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume: 5
Issue: 2
起始頁: 215
結束頁: 223
顯示於類別:期刊論文