标题: | 利用生物感测器侦测气喘生物指标-ECP与人类抗癌药物-cisplatin Biosensor for detection of asthma biomarker-ECP and anti-cancer drug-cisplatin |
作者: | 吴丽评 Wu, Li-Ping 谢有容 Hsieh, You-Zung 应用化学系硕博士班 |
关键字: | 生物感测器;电化学;萤光;嗜酸性阳离子蛋白;顺铂;biosensor;electrochemistry;fluorescence;ECP;cisplatin |
公开日期: | 2013 |
摘要: | 支气管上皮细胞上存在的硫酸乙醯肝素 (heparan sulfate) 与类似结构之肝素 (heparin) 对嗜酸性白血球阳离子蛋白 (eosinophil cationic protein,ECP) 有专一及高度的键结亲合力,实验利用肝素来当作检测ECP之探针。先简单利用定电位安培法将奈米金粒子以电沉积方式修饰在电极表面上,再使用化学合成方法,以半胱胺 (cysteamine) 为键结的桥梁,将硫酸乙醯肝素的相似物肝素藉由自组装的方式修饰至电极上,成功开发了侦测气喘生物指标 ECP 之电化学生物感测器。实验室夥伴成功将肝素修饰至金包覆铁磁性奈米粒子 (Au@Fe3O4 NPs) 上制成功能性磁奈米粒子 (Hep-Au@Fe3O4 NPs),改善电极侦测 ECP 效果。本研究进一步探讨此功能性磁奈米粒子于生物体之应用,最后证实其对细胞无毒,有结合至标靶药物开发的可能,未来可做更广泛的应用。 顺铂是治疗癌症常使用的药物,但其造成的副作用往往引起病患相当大的不适,甚至影响肝、肾功能。研究指出,顺铂与鸟嘌呤的键结会影响 DNA 双股结构的形成。实验利用人体染色体末端重复并含大量鸟嘌呤的DNA序列端粒,结合 SYBR® Green I 嵌入双股结构后萤光增强的特性,开发出简单、适用于人体血清并有高灵敏性的萤光分析方式,侦测抗癌药物顺铂之浓度,期望可应用于药物研究之领域。 Eosinophil cationic protein (ECP) was used as biomarker for asthma, which was been proved that bound with heparin and heparan sulfate existeded on cell membrane specifically. Based on the specific binding between ECP and heparin and heparan sulfate, gold nanostructured platform was simply formed on the working electrode through controlled electrodeposition, and then, the heparin was immobilized on the gold nanoparticles using the cysteamine as the linker. A electrochemical biosensor for detection of asthma biomarker was developed. other research of our lab improved the detection of ECP by immobilizing heparin on the Au@Fe3O4 NPs. This study further investigated the functional magnetic nanoparticles (Hep-Au@Fe3O4 NPs) in biological applications. We confirmed the functional magnetic nanoparticles are non-toxic and have potential to target drug development. The functional magnetic nanoparticles could be further applied into more extensively usages in the future. Cisplatin is commonly used in chemotherapy. But the side-effects it caused often makes patients discomfortable and even affects their liver and kidney function. Some studies have indicate that the binding between cisplatin and guanine will interfere the formation of DNA double-stranded structure. In this study, we used the telomere of human, a region of repetitive nucleotide sequences that contained a lot of quanine , combining with the character that performing larger fluorescence signal after binding to double-stranded DNA of SYBR ® Green.A simple strategy to sensitively detect cisplatin in human serum have developed. This method may have good prospects for drug research and development field. |
URI: | http://140.113.39.130/cdrfb3/record/nctu/#GT070152535 http://hdl.handle.net/11536/74161 |
显示于类别: | Thesis |