标题: 利用无线近红外线光谱仪进行脑组织氧分压评估
Partial Pressure Estimation of Brain Tissue Oxygen in Traumatic Brain Injury by Using Wireless Near Infrared Spectroscopy
作者: 陈昱至
Chen, Yu-Chih
林伯昰
Lin, Bor-Shyh
照明与能源光电研究所
关键字: 脑创伤;近红外线光谱仪;含氧血红素;非含氧血红素;全血红素;2,3,5氯化三苯基四氮唑;脑组织氧分压;traumatic brain injury;near-infrared spectroscopy;oxyhemoglobin;deoxyhemoglobin;totalhemoglobin;triphenyltetrazolium chloride (TTC);partial pressure of oxygen
公开日期: 2013
摘要: 在临床上,脑组织氧分压的监测对于创伤性脑损伤的患者而言是一项重要的指标。但这是一种侵入性的量测方法,且并不方便于实时的监控。近年来,近红外线光谱仪被广泛应用于神经科学,并能以非侵入性的方式评估脑部缺血及缺氧。在本论文中,利用了一个无线近红外线光谱仪和一个脑组织氧分压探测器监测大鼠的脑部在不同撞击力道的氧合状态。在液压冲击的实验中,所有大鼠被随机分配于四种不同力道之组别。并随着不同的撞击力道来观察含氧和非含氧血红素的浓度变化与脑组织氧分压之相关性。2,3,5氯化三苯基四氮唑(TTC)染色法则是用于评估脑梗死体积。结果显示,近红外线光谱仪之参数之变化量会在撞击点后立即下降后逐渐回升,而后维持于一个稳定的状态。脑组织氧分压之变化量的趋势则与之相似。TTC染色结果显示了脑梗死体积会随着撞击力道增加而上升,且含氧血红素及脑组织氧分压之变化量则随着撞击力道增加而降低。含氧血红素及脑组织氧分压之变化量间的相关系数为0.77,而非含氧血红素及脑组织氧分压之变化量间为0.08。由此可见含氧血红素之变化量与脑组织氧分压之变化量间有高的正相关性。这意味着含氧血红素之变化量可提供为参考参数来估计脑组织分压。
Monitoring partial pressure of oxygen in the brain tissue (PbtO2) is an important standard for traumatic brain injury (TBI) patients in clinical. But it is an invasive measurement and inconvenient for real-time monitoring. Recently, Near-infrared spectroscopy (NIRS) is widely used in neuroscience, and can assess cerebral ischemia and hypoxia non-invasively. In this thesis, a novel wireless NIRS system, and the PbtO2 monitoring system were used to monitor the oxygenation of rat brains under different impact strengths. And all rats were randomly assigned to four different impact strength groups in the fluid percussion injury experiments. The relationships of the concentration changes of HbO2 and HbR, and PbtO2 under and after TBI with different impact strengths were also investigated. Triphenyltetrazolium chloride (TTC) staining was used for infarction volume evaluation. Results show that Δ[HbO2], Δ[HbR], and Δ[HbT] dropped immediately after the impact and increased gradually then maintain a stable status. And Δ[PbtO2] had a similar change tendency with the NIRS parameters. The result of the TTC staining showed the infarction volume was increased with the increased impact strength, and Δ[HbO2] and Δ[PbtO2] were decreased with the increased impact strength. The correlation coefficient between Δ[PbtO2] and Δ[HbO2] is 0.77, and between Δ[HbR] and Δ[HbO2] is 0.08. It can be discovered that Δ[HbO2] is highly and positively correlated to Δ[PbtO2]. That means the Δ[HbO2] may be available to be the reference parameter to estimate the partial pressure of oxygen in the brain tissue.
URI: http://140.113.39.130/cdrfb3/record/nctu/#GT070158119
http://hdl.handle.net/11536/75329
显示于类别:Thesis