粉末冶金法製備多孔鈦支架用於間葉幹細胞分化培養之研究

Abstract

摘 要 三維培養環境的發展對於幹細胞技術的藥物發現應用和再生醫學來說相當重要，因為在三維培養條件下可以模擬幹細胞於體內的生長環境並且提供精確的分化效能。本研究探討三維培養環境對於脂肪生成以及成骨生成之分化影響；利用氯化鈉粉末作為空間支撐物，經由粉末冶金法所製備出具孔隙率變化(50-70 %)的Titanium-foam (Ti-foam)被用來作為老鼠間葉幹細胞多向分化的培養支架。即時定量PCR結果說明，三維培養環境有效促進老鼠間葉幹細胞誘導分化成脂肪細胞，提升脂肪細胞特異基因表現，包括leptin (LEP)、sterol regulatory element-binding protein 1 (SREBP-1)以及peroxisome proliferator- activated receptor γ (PPARγ)；然而，成骨生成方面並未獲得較顯著的正面效益。整體而言，Ti-foam支架為相當適合應用於誘導幹細胞分化的生醫材料，尤其於脂肪生成方面。

ABSTRACT The development of a three-dimensional (3D) culture environment is very important for drug discovery applications of stem cell technology and regenerative medicine because the 3D culture condition could mimic the stem cell environment in vivo and offer accurate differentiation. This study describes the effect of a 3D culture environment for induction of adipogenic and osteogenic differentiation. Titanium-foam (Ti-foam) with varying porosities (50-70 %) was prepared using sodium chloride (NaCl) particles as space holder through powder metallurgy (P/M) route and was used a scaffold for multilineage differentiation of rat mesenchymal stem cells (MSCs). By real-time quantitivity polymerase chain reaction (real-time qPCR), we found that our 3D culture environment efficiently promotes adipogenic differentiation of rat MSCs and adipocyte-specific gene expression, including leptin (LEP), sterol regulatory element-binding protein 1 (SREBP-1) and peroxisome proliferator-activated receptor γ (PPARγ), but not obtaining significantly positive benefits in osteogenesis. As a whole, Ti-foam is a quite suitable biomaterial for differenation of stem cell, especially in adipogenesis.