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dc.contributor.authorLiu, Jr-Chien_US
dc.contributor.authorChen, Wei-Jenen_US
dc.contributor.authorLi, Chen-Weien_US
dc.contributor.authorMong, Kwok-Kong Tonyen_US
dc.contributor.authorTsai, Pei-Janeen_US
dc.contributor.authorTsai, Te-Lungen_US
dc.contributor.authorLee, Yuan C.en_US
dc.contributor.authorChen, Yu-Chieen_US
dc.date.accessioned2014-12-08T15:10:21Z-
dc.date.available2014-12-08T15:10:21Z-
dc.date.issued2009en_US
dc.identifier.issn0003-2654en_US
dc.identifier.urihttp://hdl.handle.net/11536/7904-
dc.identifier.urihttp://dx.doi.org/10.1039/b908069den_US
dc.description.abstractPA-IL is a galactophilic lectin that is found on the outer membrane of Pseudomonas aeruginosa. Pigeon ovalbumin (POA), a phosphoprotein, contains high levels of terminal Gala(1 -> 4) Gal units. Thus, magnetic nanoparticles with immobilized POA can be used as affinity probes for P. aeruginosa, functioning via the recognition of galactophilic PA-IL. We fabricated POA-bound nanoparticles (NPs) by immobilizing POA onto the surface of core/shell magnetic iron oxide/alumina NPs via metal phosphate chelation. We then used the generated NPs to probe target bacteria from complex samples. The trapped bacterial cells were characterized based on their mass peak profiles obtained from MALDI MS analyses. In addition, we confirmed the determination of P. aeruginosa using a proteomic strategy: combining the resultant MALDI MS/MS spectra of its tryptic digest with protein database searching. The feasibility of using this approach to rapidly characterize P. aeruginosa from clinical samples without the need to perform culturing steps was also demonstrated.en_US
dc.language.isoen_USen_US
dc.titleIdentification of Pseudomonas aeruginosa using functional magnetic nanoparticle-based affinity capture combined with MALDI MS analysisen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/b908069den_US
dc.identifier.journalANALYSTen_US
dc.citation.volume134en_US
dc.citation.issue10en_US
dc.citation.spage2087en_US
dc.citation.epage2094en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000270032700018-
dc.citation.woscount13-
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