标题: 利用酪氨酸酶电化学感测器检测环境荷尔蒙
Detection of Endocrine Disrupting compounds by a Tyrosinase Biosensor
作者: 赵伟志
Wei-Chih Chao
袁俊杰
Chiun-Jye Yuan
生物科技学系
关键字: 环境荷尔蒙;电化学感测器;酪氨酸酶;Endocrine disrupt compounds (EDCs);amperometric biosensor;tyrosinase
公开日期: 2006
摘要: 内分泌干扰物,或者称之为环境荷尔蒙,这一类的化学物质存在于自然环境中或者是由工厂中所排放出,这一类的物质藉由模拟人体内正常的荷尔蒙,去与体内的荷尔蒙接受器作用,进而造成我们人体的内分泌不正常,他会直接刺激或抑制我们体内内分泌的机制,造成我们人体的一些病变,他最主要会造成的疾病有:乳癌,睾丸癌,男性精子数目下降,早熟,生殖和发育的不正常等等....。因此发展出一种快速而且简单的检测方式,便使用了酪氨酸酶(tyrosinase),藉由包埋的方式固定在碳胶电极(SPCE)的表面上形成一个电流式的生物感测器,藉由电化学的方法来做为我们的检测方式。Bisphenol A, diethylstilbestrol, paraben 和 3-aminophenol在文献中说明了它们具有环境荷尔蒙的性质,因此便拿来做为检测的物质。在电化学实验中所施加的工作电位为-0.2V,结果所得到的检量线范围分别为0.1 ~ 20 μM, 10 ~ 100 μM, 1 ~ 50 μM和 20 μM而侦侧极限为20 nM, 0.4 μM, 20 nM and 5 μM分别对Bisphenol A, diethylstilbestrol, paraben 和 3-aminophenol我们所用来做检测的感测器它具有良好的连续性测试再现性(R.S.D = 5.8%)。
Endocrine disrupt compounds (EDCs) or environmental hormones are steroid-like compounds that exist in nature or are produced during industrial process. EDCs, once ingested, may interfere with the functions of normal endocrine system in both wildlife and humans. Hence, the development of a quick and easy operating biosensing device is necessary. In this study, an amperometric biosensor for the detection of endocrine disrupt compounds based on tyrosinase entrapped within gelatin film is designed and developed. The suspected endocrine disrupt compounds, including bisphenol A, diethylstilbestrol (DES), paraben and 3-aminophenol, were tested on the developed biosensor. Amperometric response of biosensor to EDCs in a phosphate buffer at pH 6 was measured as a function of concentrations at fixed bias voltage of −0.2 V versus Ag/AgCl. The electrode gives linear response ranges of 0.1 ~ 20 μM, 10 ~ 100 μM, 1 ~ 50 μM and 20 ~ 100 μM for bisphenol A, diethylstilbestrol (DES), 3-aminophenol and paraben , respectively. The detection limit for bisphenol A, diethylstilbestrol (DES), 3-aminophenol and paraben are 20 nM, 0.4 μM, 20 nM and 5 μM, respectively. Moreover, the developed biosensor exhibits a good reproducibility with a relative standard devision (R.S.D) of 5.8 %.
URI: http://140.113.39.130/cdrfb3/record/nctu/#GT009428518
http://hdl.handle.net/11536/81497
显示于类别:Thesis


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