生長激素-奈米鑽石複合體的開發與其應用

Abstract

奈米科技是一門嶄新的科學，其主要研究奈米材料的基礎特性及應用性。當任一塊材物質逐漸的縮小其某一維度的尺寸到達100 nm以下，即成為所謂的奈米材料，而奈米粒子就是其中的一種。相較於一般塊材，奈米材料具有極為不同的物理及化學特性，奈米鑽石亦不例外。早期的奈米鑽石多用於機械上的工業加工或是表面處理，但有研究學者發現奈米鑽石可能具有螢光特性，因此本研究對奈米鑽石做詳加的研究，最後察覺奈米鑽石是具有螢光特性的奈米材料。也有其他實驗指出奈米鑽石可能不具有細胞毒性，因此奈米鑽石是否具有跨足生醫領域的潛能並將於本研究中詳加探討。在本實驗中，我們利用細胞活性實驗證實奈米鑽石(nanodiamond, ND)是無毒材料，並藉由其螢光特性察覺奈米鑽石具有細胞穿透性，可出現於細胞質及細胞核中。因此我們認為奈米鑽石可做為攜帶生物分子的載體的潛能，或是開發出具有生物標靶性的蛋白質-奈米鑽石複合體。本研究首先藉由比較不同物種間的親源關係來找出人類生長激素的類似物EaGH，並利用基因重組技術取得大量的基因重組青石斑魚生長激素(rEaGH)作為活性標定奈米鑽石的生物分子。藉由基質輔助雷射脫附游離時序飛行質譜儀(MALDI-TOF)的分析得知一個奈米鑽石和兩個rEaGH形成一個共價結合的cND-rEaGH複合體。而細胞活性測試結果顯示rEaGH、ND和cND-rEaGH複合體都不具有細胞毒性，然而一但結合雷射系統與對生長激素受體具有標靶性的cND-rEaGH複合體，將對癌症細胞產生爆炸性的損害，我們利用掃描式電子顯微鏡可觀察到奈米鑽石的爆炸威力對細胞的損害程度，必藉由細胞活性測試量實驗量化細胞的受損情形。最終本實驗成功的藉由結合具有肺癌標靶性的cND-rEaGH複合體和雷射系統，建立一治療肺癌的醫療平台。

Nanomaterial is a materiall which size is smaller than hundred nanometers. Nanomaterials possess unique chemical and physical propertyes and they are different from bulk materials. Nano-diamond (ND) is one of the unique example. In general, ND was used as grind powder in processing industries. Recently, we found that ND may possess unique fluorescence properties. Meanwhile we also found that ND may be bio-compatible particle. This material may be a good candidate in bio-medical application. Therefore in this study, we have characterized the intrinsic properties of ND and carboxyl-ND. We found that ND is a non-toxic material for bio-system. Meanwhile, NDs can be uptake freely by cell and these ND particles can be observed by fluorescence microscopy. We have synthesized a GH-ND complex and we found that this complex can be recognized by the GH receptors which located on the cell surface. Therefore, we found that these complex can be triggered to blast on the surface of cancer cell. Therefore, we may conclude that the GH-ND complex may be used in biomedical application, such as cancer therapy. Meanwhile this nanoparticle may be used as a bio-compatible platform in many kind application of bio-system.