標題: | Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid |
作者: | Chen, Yi-Pei Chen, I-Ching Hwang, Ing-Er Yuan, Gwo-Fang Liaw, Li-Ling Tseng, Ching-Ping 生物科技學系 Department of Biological Science and Technology |
關鍵字: | Monascus pilosus;restriction-enzyme-mediated integration (REMI);aurintricarboxylic acid (ATA) |
公開日期: | 1-十一月-2008 |
摘要: | The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation. |
URI: | http://dx.doi.org/10.1271/bbb.80342 http://hdl.handle.net/11536/8162 |
ISSN: | 0916-8451 |
DOI: | 10.1271/bbb.80342 |
期刊: | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY |
Volume: | 72 |
Issue: | 11 |
起始頁: | 3021 |
結束頁: | 3024 |
顯示於類別: | 期刊論文 |